||Recombinant human PDRG1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. MW = 17.6 kDa (153aa) confirmed by MALDI-TOF.
||PDRG1, also known as PDRG or C20orf126, is a 133 amino acid protein that localizes to the cytoplasm and belongs to the prefoldin subunit β family. Expressed predominately in normal testicular tissue, PDRG1 is functionally induced by ultraviolet light and is thought to play a role in chaperone-mediated protein folding, possibly playing a role in cellular degradation. It is associated with Creutzfeldt-Jakob disease, amyotrophic lateral sclerosis, spinal muscular atrophy, ring chromosome 20 epilepsy syndrome and Alagille syndrome.
|Sequences Of Amino Acids:
||MGSSHHHHHH SSGLVPRGSH MLSPEAERVL RYLVEVEELA EEVLADKRQI VDLDTKRNQN REGLRALQKD LSLSEDVMVC FGNMFIKMPH PETKEMIEKD QDHLDKEIEK LRKQLKVKVN RLFEAQGKPE LKGFNLNPLN QDELKALKVI LKG
||> 90% by SDS – PAGE.
||Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 5mM DTT, 20% glycerol.
||0.5 mg/ml (determined by Bradford assay).
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.