||Recombinant human ANP32A protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. MW = 30.7kDa (269aa), confirmed by MALDI-TOF.
||ANP32A (acidic leucine-rich nuclear phosphoprotein 32 family member A) has been shown to interact with MAP1B, TAF1A and Protein SET. This proteinImplicated in a number of cellular processes, including proliferation, differentiation, caspase-dependent and caspase-independent apoptosis. Also, it plays a role in E4F1-mediated transcriptional repression.
|Sequences Of Amino Acids:
||MGSSHHHHHH SSGLVPRGSH MEMGRRIHLE LRNRTPSDVK ELVLDNSRSN EGKLEGLTDE FEELEFLSTI NVGLTSIANL PKLNKLKKLE LSDNRVSGGL EVLAEKCPNL THLNLSGNKI KDLSTIEPLK KLENLKSLDL FNCEVTNLND YRENVFKLLP QLTYLDGYDR DDKEAPDSDA EGYVEGLDDE EEDEDEEEYD EDAQVVEDEE DEDEEEEGEE EDVSGEEEED EEGYNDGEVD DEEDEEELGE EERGQKRKRE PEDEGEDDD
||> 90% by SDS – PAGE.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 1mM DTT, 100mM NaCl, 0.1mM PMSF.
||0.5mg/ml (determined by Bradford assay).
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.