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Recombinant Human Tec Protein Tyrosine Kinase, GST-tagged

Cat. No.: TEC-2621H
Product Overview: Recombinant Human TEC, catalytic domain [359-631 amino acids] was expressed as N-terminal GST-fusion protein (59 kDa) usingbaculovirusexpression system. GST-TEC was purified by using glutathione sepharose chromatography and anion exchange chromatography.
Description: The protein encoded by this gene belongs to the Tec family of non-receptor protein-tyrosine kinases containing a pleckstrin homology domain. Tec family kinases are involved in the intracellular signaling mechanisms of cytokine receptors, lymphocyte surface antigens, heterotrimeric G-protein coupled receptors, and integrin molecules. They are also key players in the regulation of the immune functions. Tec kinase is an integral component of T cell signaling and has a distinct role in T cell activation. This gene may be associated with myelodysplastic syndrome.
Source: baculovirus.
Purity: 100%. The purity was assessed by SDS-PAGE/CBB staining.
Storage Buffer: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5.
ATP: 100μM.
Substrate: Srctide.
Storage: Store at -80℃. Avoid repeating freeze-thaws.
Gene Name: TEC tec protein tyrosine kinase [ Homo sapiens ]
Synonyms: TEC; tec protein tyrosine kinase; PSCTK4; MGC126760; MGC126762; tyrosine-protein kinase Tec; EC 2.7.10.2
Gene ID: 7006
mRNA Refseq: NM_003215
Protein Refseq: NP_003206
MIM: 600583
UniProt ID: P42680
Chromosome Location: 4p12
Pathway: T cell receptor signaling pathway
Function: ATP binding; metal ion binding; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; protein binding; transferase activity

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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