||Recombinant Human TEC, catalytic domain [359-631 amino acids] was expressed as N-terminal GST-fusion protein (59 kDa) usingbaculovirusexpression system. GST-TEC was purified by using glutathione sepharose chromatography and anion exchange chromatography.
||The protein encoded by this gene belongs to the Tec family of non-receptor protein-tyrosine kinases containing a pleckstrin homology domain. Tec family kinases are involved in the intracellular signaling mechanisms of cytokine receptors, lymphocyte surface antigens, heterotrimeric G-protein coupled receptors, and integrin molecules. They are also key players in the regulation of the immune functions. Tec kinase is an integral component of T cell signaling and has a distinct role in T cell activation. This gene may be associated with myelodysplastic syndrome.
||100%. The purity was assessed by SDS-PAGE/CBB staining.
||50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5.
||Store at -80℃. Avoid repeating freeze-thaws.