|Cat. No. :
|Product Overview :
||Recombinant Human interferon-beta-1b (IFN-β-1b) produced in Pseudomonas fluorescens contains a Cys17Ser substitution; and after expression, lacks the N-terminal methionine. MW = 19.9 kDa.
||Human interferon beta (IFN-β) is produced in many cell types in response to viral infection, microorganisms, double-stranded RNA, and certain cytokines. IFN-β is known to regulate genes involved in antiviral, antiproliferative, and immunomodulatory activities. Endogenously expressed IFN-β-1a is a glycoprotein; however, nonglycosylated versions have demonstrated similar activities.
|N-terminal Sequence :
||>97%, by SDS-CGE under reducing condition.
||Lyophilized with sucrose and glycine.
|Endotoxin Level :
||<1.0 EU per 1 μg of the protein by the LAL method.
||Measured in a viral inhibition (CPE) assay using EMC virus on A549 cells. Measured in an interferon receptor-2 binding assay.
||Store at 2-8°C upon receipt, preferably desiccated. Reconstitute aseptically in sterile 20 mM acetic acid, pH 4.0, to a final IFN-β concentration of 0.15-0.25 mg/mL. A carrier (such as albumin) is recommended if further dilution is needed. Store reconstituted IFN-β at -20°C (not in a frost-free freezer). Avoid repeated freeze-thaw.