||Recombinant Human STK4, amino acids M3-F487, Nterminal GST-HIS6 fusion protein with a 3C cleavage site, was expressed inSf9insect cells. MW =86,340 Da.
||The protein encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it"s possible that this protein induces the chromatin condensation observed in this process.
||Baculovirus infected Sf9 cells.
||MST1 was confirmed as MST1 by mass spectroscopy LC-ESI-MS/MS.
||50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol.
||0.306 μg/μl (Bradford method using BSA [Sigma, cat# A-7638, Lot 79H7641] as standard protein).
||-80°C. Avoid repeated freeze-thaw cycles!