||Recombinant Human PAK7, Amino acids M1-H719, N-terminally fused to GST-HIS6-Thrombin cleavage site, was expressed inSf9cells. MW = 110.660 Da.
||PAK family members are known to be effectors of Rac/Cdc42 GTPases, which have been implicated in the regulation of cytoskeletal dynamics, proliferation, and cell survival signaling. This kinase contains a CDC42/Rac1 interactive binding (CRIB) motif, and has been shown to bind CDC42 in the presence of GTP. This kinase is predominantly expressed in brain. It is capable of promoting neurite outgrowth, and thus may play a role in neurite development.
||Baculovirus infected Sf9 cells.
||One-step affinity purification using GSH-agarose.
||PAK7 was confirmed as human PAK7 by mass spectroscopy LC-ESI-MS/MS (Protagen AG, Germany).
||50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4mM reduced glutathione, 20% glycerol.
||0.152 μg/μl (Bradford method using BSA [Sigma, cat# A-7638, Lot 79H7641] as standard protein).
||-80°C. Avoid repeated freeze-thaw cycles!