Recombinant Human PAK7, GST-His

• Cat.No.: PAK7-725H
• Product Overview: Recombinant Human PAK7, Amino acids M1-H719, N-terminally fused to GST-HIS6-Thrombin cleavage site, was expressed inSf9cells. MW = 110.660 Da.

Specification

• Cat. No.: PAK7-725H
• Description: PAK family members are known to be effectors of Rac/Cdc42 GTPases, which have been implicated in the regulation of cytoskeletal dynamics, proliferation, and cell survival signaling. This kinase contains a CDC42/Rac1 interactive binding (CRIB) motif, and has been shown to bind CDC42 in the presence of GTP. This kinase is predominantly expressed in brain. It is capable of promoting neurite outgrowth, and thus may play a role in neurite development.
• Source: Baculovirus infected Sf9 cells.
• Purification: One-step affinity purification using GSH-agarose.
• Product Identity: PAK7 was confirmed as human PAK7 by mass spectroscopy LC-ESI-MS/MS (Protagen AG, Germany).
• Storage Buffer: 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4mM reduced glutathione, 20% glycerol.
• Concentration: 0.152 μg/μl (Bradford method using BSA [Sigma, cat# A-7638, Lot 79H7641] as standard protein).
• Specific Activity: 18 pmol/μg×min.
• Storage: -80°C. Avoid repeated freeze-thaw cycles!

Gene Information

• Gene Name: PAK7 p21 protein (Cdc42/Rac)-activated kinase 7 [ Homo sapiens ]
• Synonyms: PAK7; p21 protein (Cdc42/Rac)-activated kinase 7; PAK5; KIAA1264; MGC26232; OTTHUMP00000030260; p21(CDKN1A)-activated kinase 7; p21-activated kinase 7; p21CDKN1A-activated kinase 7; protein kinase PAK5; serine/threonine-protein kinase PAK7; EC 2.7.11.1
• Gene ID: 57144
• mRNA Refseq: NM_020341
• Protein Refseq: NP_065074
• MIM: 608038
• UniProt ID: Q9P286
• Chromosome Location: 20p12
• Pathway: Axon guidance; ErbB signaling pathway; Focal adhesion; Regulation of actin cytoskeleton; Renal cell carcinoma; T cell receptor signaling pathway
• Function: ATP binding; nucleotide binding; protein binding; protein serine/threonine kinase activity; transferase activity