||RecombinantHuman PIM2, Amino acids M1-P311, N-terminally fused to GST-HIS6-Thrombin cleavage site, was expressed inSf9cells. MW = 64,096 Da.
||PIM2 is a scoring system for rating the severity of medical illness for children, one of several ICU scoring systems. Its name stands for "Paediatric Index of Mortality". It has been designed to provide a predicted mortality for a patient by following a well defined procedure. Predicted mortalities are good when dealing with several patients, because the average predicted mortality for a group of patients is an indicator for the morbidity of these patients.
||Baculovirus infected Sf9 cells.
||One-step affinity purification using GSH-agarose.
||PIM2 was confirmed as PIM2 by specific Western Blotting using anti PIM2 antibody.
||50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol.
||0.216 μg/μl (Bradford method using BSA [Sigma, cat# A-7638, Lot 79H7641] as standard protein).
||-80°C. Avoid repeated freeze-thaw cycles!