||Recombinant Human TTK, full length, amino acids M1-K857, N-terminal GST-HIS6 fusion protein with a Thrombin cleavage site, was expressed inSf9insect cells. MW = 126,968 Da.
||Dual specificity protein kinase TTK is an enzyme that in humans is encoded by the TTK gene.
||Baculovirus infected Sf9 cells.
||TTK was confirmed as TTK by mass spectroscopy LC-ESI-MS/MS (Protagen AG, Germany)
||50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol.
||0.064 μg/μl (Bradford method using BSA [Sigma, cat# A-7638, Lot 79H7641] as standard protein).
||-80°C. Avoid repeated freeze-thaw cycles!