Recombinant Rat Epidermal Growth Factor

Cat. No.: Egf-460R
Product Overview: Recombinant Rat Epidermal Growth Factor is expressed inE. coli.
Description: EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF-α and VGF (vaccinia virus growth factor). Recombinant rat EGF is a 6.2 kDa globular protein containing 54 amino acid residues including 3 intramolecular disulfide-bonds.
Source: E. coli.
Specific Activity: ≥ 1 x 107units/mg.
Endotoxin Level: < 0.1 ng per µg of rRa EGF (1EU/µg).
Purity: Greater than 98% (determined by SDS-PAGE and HPLC analysis).
Biological Activity: The ED50was determined by a cell proliferation assay using BALB/c 3T3 cells is < 0.1 ng/ml, corresponding to a specific activity of > 1 x 107units/mg.
Formulation: Lyophilized from a sterile-filtered solution with no additives.
Reconstitution: Please Note: Always centrifuge product before opening vial. The lyophilized protein should be reconstituted with distilled, sterile water to a concentration of 0.1-1.0 mg/ml. For further dilution, carrier protein (0.1% BSA or HSA) should be added to avoid loss of bioactivity.
Storage & Stability: The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Gene Name: Egf epidermal growth factor [ Rattus norvegicus ]
Synonyms: Egf; epidermal growth factor (beta-urogastrone); URG; HOMG4; Pro-epidermal growth factor; Epidermal growth factor; Urogastrone
Gene ID: 25313
mRNA Refseq: NM_012842
Protein Refseq: NP_036974
UniProt ID: P07522
Chromosome Location: 2q42-q43
Pathway: Bladder cancer; Cytokine-cytokine receptor interaction; Endocytosis; Endometrial cancer; ErbB signaling pathway; Focal adhesion; Gap junction; Glioma; MAPK signaling pathway; Melanoma; Non-small cell lung cancer; Melanoma; Non-small cell lung cancer; Pancreatic cancer; Pathways in cancer; Prostate cancer; Regulation of actin cytoskeleton
Function: calcium ion binding; epidermal growth factor receptor binding; growth factor activity; protein binding

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