||Recombinant human His tagged LXR-β is isolated from anE. colistrain that carries the coding sequence of the human LXR-β under the control of a T7 promoter.62 kDa.
||Liver X receptors (LXRs) are nuclear receptors that regulate the metabolism of cholesterol and bile acids. There are two subtypes of LXRs, LXRα and LXR-β. LXR-β is preferentially expressed in liver, small intestine, kidney and spleen. In contrast, LXRα expression is ubiquitous. The genomic structure and the promoter regions of the two LXR genes contain specific regulatory sites, which suggest that LXRs may have physiological roles in the immune system. Like other nuclear receptors, LXRs heterodimerize with retinoid X receptor (RXR) for function. LXRs are activated by naturally occurring oxysterols and regulate the expression of target genes, including ATP binding cassette transporter 1 (ABC1), ATP binding cassette transporter 8 (ABC8) and cholesterol ester transfer protein (CETP). LXR-β expressed in livers of LXRα knockout mice does not compensate for the loss of LXRα. In addition, LXR-β, but not LXRα, is also able to activate transcription of a reporter gene, which contains a specific direct repeat separated by 1 bp (DR1) element in the promoter, suggesting that LXR-β may have different biological functions.
||LXR-β can be used for DNA and protein-protein interactions assays.
||1 unit equals 1 nanogram of purified protein. 20 units are sufficient for a gel-mobility shift assay and 100 units are sufficient for a protein-protein interaction assay.
||The purified recombinant protein is greater than 95% homogeneous based on SDS-PAGE analysis.
||1x dilution buffer A: 20 mM Tris-Cl (pH 7.9), 20% Glycerol, 100 mM KCl, 0.2 mM EDTA and 1 mM DTT.
||Store at -80°C.