| Cat. No. : |
RXRB-2514H |
| Description : |
Retinoid X receptor (RXR) serves as a promiscuous heterodimerization partner for many nuclear receptors through the identity box, a 40-amino acid subregion within the ligand binding domain. RXR partners include thyroid hormone receptors (TRs), retinoic acid receptors (RARs), peroxisome proliferator-activated receptor (PPAR), several constitutive active orphan nuclear receptors, e.g. nuclear growth factor I-B (NGFI-B), oxysterol receptors (FXR,LXR), and constitutive androstane receptors (CAR). RXRs also form homodimers to mediate the effects of 9-cis-retinoic acid (9-cRA). Depending on these protein-protein interactions, RXR-containing complexes have distinct ligand-dependent and constitutive functions. RXR-β was originally identified as a protein able to bind to the regulatory region II of the murine major histocompatibility complex (MHC) class I promoter and referred to as H2RIIBP. The human RXR-β gene has been mapped to the short arm or centromeric region of chromosome 6 (6pter-q13) which also contains the MHC I locus. Two major RXR-b isoforms in the mouse, designated RXR-β1 and RXR-β2, differ in the N-terminal (A) domain with RXR-β1 containing an extra 72 amino acids. These isoform mRNAs are transcribed from two CpG island promoters and the different N-terminal exons are spliced to exons common to both isoforms from the remainder of the gene. Although the two mouse RXR isoforms have been characterised in some detail, less information is available about their human counterparts. |
| Source : |
baculovirus. |
| Applications : |
RXRβ can be applied to in vitro transcription assays, DNA and protein-protein interactions assays. |
| Activity : |
1 unit equals 1 nanogram purified protein. 20 units (ng) are sufficient for an in vitro transcription assay and 100 units are sufficient for a protein-protein interaction assay. |
| Quality Control : |
The purified protein is greater than 95% homogeneous based on SDS-PAGE gel analysis. |
| Reagents Supplied : |
1x dilution buffer A: 20 mM Tris-Cl (pH 8.0), 20% Glycerol, 100 mM KCl, 0.2 mM EDTA and 1mM DTT. |
| Storage Conditions : |
Store at -80°C. |
| Pathways : |
Adipocytokine signaling pathway; Non-small cell lung cancer; PPAR signaling pathway; Pathways in cancer; Small cell lung cancer; Thyroid cancer |
