Recombinant Human UBE2A, His-tagged UBE2A-53H

Recombinant Human UBE2A, His-tagged

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Recombinant Human UBE2A, His-tagged

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Cat. No. : UBE2A-53H
Product Overview : Recombinant human UBE2A, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW = 19.4 kDa (172aa).
Description : Ubiquitin-conjugating enzyme E2 A isoform 1, also known as UBE2A is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Multiple alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms.
Source : E.coli.
Form : Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 1mM EDTA.
Purity : > 95% by SDS - PAGE.
Concentration : 0.5mg/ml (determined by Bradford assay).
Sequences Of Amino Acids : MGSSHHHHHH SSGLVPRGSH MSTPARRRLM RDFKRLQEDP PAGVSGAPSE NNIMVWNAVI FGPEGTPFED GTFKLTIEFT EEYPNKPPTV RFVSKMFHPN VYADGSICLD ILQNRWSPTY DVSSILTSIQ SLLDEPNPNS PANSQAAQLY QENKREYEKR VSAIVEQSWR DC.
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
PDB rendering based on 1jas. :

Gene Name : UBE2A ubiquitin-conjugating enzyme E2A (RAD6 homolog) [ Homo sapiens ]
Synonyms : ubiquitin-conjugating enzyme E2A (RAD6 homolog); UBC2; HHR6A; RAD6A; UBE2A; ubiquitin-conjugating enzyme E2 A; HR6A; RAD6 homolog A; ubiquitin-protein ligase A; ubiquitin carrier protein A; Ubiquitin-protein ligase A; Ubiquitin carrier protein A; EC 6.3.2.19; hHR6A
Gene ID : 7319
mRNA Refseq : NM_003336
Protein Refseq : NP_003327
MIM : 312180
UniProt ID : P49459
Chromosome Location : Xq24-q25
Pathway : Ubiquitin mediated proteolysis
Function : ATP binding; ligase activity; nucleotide binding; protein binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity

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