"NEK6" Related Products

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Recombinant Human NEK6, Active

Cat. No. : NEK6-662H
Description : NEK6 is a serine/threonine kinase that is a member of the Nek family of protein kinases that share an amino-terminal catalytic domain related to NIMA family. Nek6 is a nuclear and cytoplasmic kinase that is required for mitotic progression of human cells. Nek6 is phosphorylated and activated during M phase of the cell cycle. Inhibition of Nek6 function by either overexpression of an inactive Nek6 mutant or elimination of endogenous Nek6 by siRNA arrests cells in M phase and triggers apoptosis.
Source : E.coli.
Application : Kinase activity is measured as the molar amount of phosphate incorporated into myelin basic protein, per minute per mg protein at 30°C using a final concentration of 50 μM [32P] ATP.
Biochem/physiol Actions : Nek6 is a nuclear and cytoplasmic kinase that is required for mitotic progression of human cells. It is phosphorylated and activated during M phase of the cell cycle. Inhibition of Nek6 arrests cells in M phase and triggers apoptosis, suggesting that Nek6 is required for metaphase-anaphase transition.
Physical Form : Solution in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, and 30% glycerol.
Assay : ≥80% (SDS-PAGE).
Form : buffered aqueous glycerol solution.
Mol Wt : protein apparent mol wt ~58 kDa.
Storage Temp : −70°C.
Gene Name : NEK6 NIMA (never in mitosis gene a)-related kinase 6 [ Homo sapiens ]
Synonyms : NEK6; NIMA (never in mitosis gene a)-related kinase 6; SID6-1512; NIMA-related kinase 6; OTTHUMP00000022097; putative serine-threonine protein kinase; EC 2.7.11.1
Gene ID : 10783
mRNA Refseq : NM_001145001
Protein Refseq : NP_001138473
MIM : 604043
UniProt ID : P51955
Chromosome Location : 9q33.3-q34.11
Function : ATP binding; magnesium ion binding; nucleotide binding; protein phosphatase binding; protein serine/threonine kinase activity; transferase activity; protein serine/threonine kinase activity; signal transducer activity

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