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Recombinant Human ABCC2

Cat. No.: ABCC2-2537H
Product Overview: Recombinant Human ABCC2 was produced inSf9 cells.
Description: MRP2 (ABCC2) is an organic anion transporter found in the liver, kidney, and gut epithelium apical membranes. The transport of glucuronate conjugates plays a role in the detoxification of endogenous and xenobiotic substances, and may cause multidrug resistance (MDR) in tumor cells.
Source: Sf9 cells.
Form: Supplied as isolated Sf9 cell membranes containing human MRP2 suspended in TMEP solution.
Biochem/physiol Actions: The vesicular transport assay determines the interaction of compounds with the MRP2 transporter. The interaction is detected by changes in the initial rate of 3H-β-estradiol 17-(β-D-glucuronide) transport by MRP2 into membrane vesicles purified from Sf9 cells expressing the transporters. Membrane preparations from infected cells always contain some closed membrane vesicles that have an inside-out orientation (5-10% of total lipid). In the case of these inside-out vesicles, transport of substrates across the membrane takes molecules from the surrounding buffer and transports them into the vesicles.
Analysis Note: The quantity of transported molecules can be determined by methods such as HPLC, LC/MS/MS separation and detection, and also by labeling with fluorescent or radioactive (3H-β-estradiol 17-(β-D-glucuronide) tags. MRP2 mediates the transport of β-estradiol 17-(β-D-glucuronide) (E217βG) very efficiently. Compounds that interact with the transporter modulate the initial rate of E217βG transport measured without any other compounds added. If a substance is a transported substrate of the transporter, it might compete with E217βG, thus reducing the rate of E217βG transport. If a compound is an inhibitor of the transporter, it will block the transport of E217βG into the membrane vesicles. Some compounds can be co-transported with E217βG increasing the rate of E217βG transport compared to the control level.
Storage: −70°C.
Gene Name: ABCC2 ATP-binding cassette, sub-family C (CFTR/MRP), member 2 [ Homo sapiens ]
Synonyms: ABCC2; ATP-binding cassette, sub-family C (CFTR/MRP), member 2;ABC30; CMOAT; DJS; KIAA1010; MRP2; cMRP; OTTHUMP00000020267;canalicular multispecific organic anion transporter; Canalicular multispecific organic anion transporter 1;Multidrug resistance-associated protein 2; Canalicular multidrug resistance protein; CMOAT1; ATP-binding cassette sub-family C member 2
Gene ID: 1244
mRNA Refseq: NM_000392
Protein Refseq: NP_000383
MIM: 601107
UniProt ID: Q92887
Chromosome Location: 10q24
Pathway: ABC transporters - General
Function: ATP binding; ATPase activity; coupled to transmembrane movement of substances;nucleotide binding; organic anion transmembrane transporter activity; protein binding; transporter activity

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