Recombinant Rat Interleukin 33

• Cat.No.: Il33-286R
• Product Overview: Recombinant rat IL-33 (aa 109-264) was expressed in E. coli, ~16kDa.

Specification

• Cat. No.: Il33-286R
• Description: IL-33 is a new member of the IL-1 family. It is involved in T helper type 2 response via the activation of its receptor ST2 (IL-1RL1) driving the production of Th2-associated cytokines. The stimulation of ST2 by IL-33 induces the NF-κB and MAPK pathways. The human IL-33 protein is expressed with a prodomain (aa 1-111) which is cleaved by caspase-1 to produce the 18kDa mature protein (112-270).
• Source: E. coli.
• Purity: ≥95% (SDS-PAGE).
• Concentration: 0.1mg/ml after reconstitution.
• Formulation: Lyophilized. Contains PBS.
• Endotoxin Content: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
• Reconstitution: Reconstitute with 100µl sterile distilled water. Further dilutions should be made with medium containing 5% fetal calf serum.
• Long Term Storage: -20°C.
• Use/Stability: Stable for at least 6 months after receipt when stored at -20°C.
• Handling: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Gene Information

• Gene Name: Il33 interleukin 33 [ Rattus norvegicus ]
• Synonyms: interleukin 33; RGD1311155; Il33; interleukin-33; IL-33
• Gene ID: 361749
• mRNA Refseq: NM_001014166
• Protein Refseq: NP_001014188
• UniProt ID: Q66H70
• Chromosome Location: 1q52
• Pathway: Cytosolic DNA-sensing pathway
• Function: cytokine activity; protein binding