Recombinant Human Intelectin 1 (Galactofuranose Binding), FLAG-tagged

• Cat.No.: ITLN1-2176H
• Product Overview: Recombinant Human Intelectin 1 produced inHEK 293was fused at the N-terminus to a FLAG®-tag. Polypeptide chain containing 142 amino acids and having a molecular mass of 30-40 kDa.

Specification

• Cat. No.: ITLN1-2176H
• Description: Omentin 1 (also named intelectin-1, endothelial lectin HL-1 and intestinal lactoferrin receptor) has been recently identified as a novel visceral fat (or omental fat) depot-specific adipokine. Omentin 1 is a secreted protein of 38kDa containing a fibrinogen-related domain. Omentin 1 is highly expressed in the omental fat, and much less in intestine, lung and heart. Addition of recombinant omentin in vitro did not affect basal, but enhanced insulin-stimulated glucose uptake in subcutaneous as well as in omental human adipocytes. Omentin 1 triggers AKT signalling in the absence of insulin.
• Source: HEK 293 cells.
• Concentration: 0.1mg/ml after reconstitution.
• Purity: ≥95% (SDS-PAGE)
• Formulation: Lyophilized. Contains PBS.
• Endotoxin Content: <0.1EU/µg purified protein.
• Reconstitution: Reconstitute with 100µl sterile water. PBS containing at least 0.1% BSA should be used for further dilutions.
• Biological Activity: Enhances insulin-mediated glucose transport in 3T3-L1 adipocytes.
• Storage: Short-term storage: +4°C. Long-term storage: -20°C.
• Stability: Stable for at least 6 months after receipt when stored at -20°C.
• Handling: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

Gene Information

• Gene Name: ITLN1 intelectin 1 (galactofuranose binding) [ Homo sapiens ]
• Synonyms: ITLN1; intelectin 1 (galactofuranose binding); HL1; LFR; HL-1; INTL; ITLN; hIntL; omentin; FLJ20022; Intelectin-1; Omentin; Galactofuranose-binding lectin; Intestinal lactoferrin receptor; Endothelial lectin HL-1; OTTHUMP00000027882
• Gene ID: 55600
• mRNA Refseq: NM_017625
• Protein Refseq: NP_060095
• MIM: 609873
• UniProt ID: Q8WWA0
• Chromosome Location: 1q21.3
• Function: receptor binding; sugar binding

Reviews

12/29/2021

ITLN1 activity is good, and the required biological activity experiments can be carried out effectively.

12/13/2021

The results show that the protein has good crystallinity, which is helpful for crystallographic study.

07/20/2021

Its high solubility allows for easy preparation of the required concentration.

Q&As

What are the directions worth paying attention to for ITLN1 in future research?

In future studies, it is worth paying attention to the relationship between structure and function, expression regulation, association with other proteins or diseases, and therapeutic potential. At the same time, further research is needed on the similarities and differences between them and other protein inhibitors, as well as their differences in different species.

How about the ITLN1 expression regulated?

The expression level of ITLN1 is regulated by a variety of factors, including processes such as gene transcription, translation, and post-translational modifications, as well as hormones, nutrients, and other environmental factors in the body.

Does ITLN1 have other biological activities?

Yes, ITLN1 may have other biological activities in addition to trypsin inhibitory activity. For example, it may play a role in processes such as immune regulation, cell growth, and apoptosis.

Are there differences in ITLN1 among different species?

Yes, there may be some differences in ITLN1 in different species, such as different degrees of variation in structure, function, and expression levels.

How does the synthesis and secretion of ITLN1 in the human body take place?

The synthesis and secretion of ITLN1 in the human body involves multiple steps and complex regulatory mechanisms, including gene transcription, translation, post-translational modification and secretion.

What are the similarities and differences between ITLN1 and other protein inhibitors?

There are certain similarities and differences between ITLN1 and other protein inhibitors in terms of structure, function, and mechanism of action. For example, other protein inhibitors may have different mechanisms of inhibition and ranges of action.