Recombinant Human SERPINA3 protein, His-tagged
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|Product Overview :||Recombinant Human SERPINA3 (His24-Ala423) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).|
|Description :||Serpin A3 is also known as Alpha-1-antichymotrypsin (ACT or AACT), Cell growth-inhibiting gene 24/25 protein, which belongs to the serpin family. Serpin A3 can bind DNA. Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2.|
|Predicted N Terminal :||His24|
|Form :||Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.|
|Molecular Mass :||The protein has a calculated MW of 46.6 kDa. The protein migrates as 50-63 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.|
|Protein length :||His24-Ala423|
|Endotoxin :||Less than 1.0 EU per μg by the LAL method.|
|Purity :||>95% as determined by SDS-PAGE.|
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.|
|SDS-PAGE of SERPINA3-665H :||
Human Serpin A3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
|Gene Name :||SERPINA3|
|Official Symbol :||SERPINA3|
|Synonyms :||SERPINA3; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3; AACT, alpha 1 antichymotrypsin , serine (or cysteine) proteinase inhibitor, clade A (alpha 1 antiproteinase, antitrypsin), member 3; alpha-1-antichymotrypsin; ACT; alpha 1 antichymotrypsin; serpin A3; growth-inhibiting protein 24; growth-inhibiting protein 25; cell growth-inhibiting gene 24/25 protein; serine (or cysteine) proteinase inhibitor, clade A, member 3; AACT; GIG24; GIG25; MGC88254|
|Gene ID :||12|
|mRNA Refseq :||NM_001085|
|Protein Refseq :||NP_001076|
|UniProt ID :||P01011|
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◆ Native Protein
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