Dual Luciferase Reporter Gene Assay Kit
Cat.No. : | Kit-2422 |
Product Overview : | The accuracy of reporter assays can be improved by utilizing a dual reporter system. One of the reporter genes is correlated with the promoter of interest and is used to assess the effects of specific experimental conditions, while the second reporter is used as a control and serves as a baseline response. The Dual-Luciferase Reporter Gene Assay allows for the sequential measurement of the activity of two different luciferases, firefly (FFL) and Renilla (RL), in a single sample. The firefly luciferase luminescence is measured first by addition of the FFL Reagent. Next, the RL Reagent is added to the same well. The RL Reagent simultaneously quenches the firefly luciferase luminescence and initiates the Renilla luciferase reaction. The light production of both reactions can be conveniently measured on a luminometer. This bioluminescent dual reporter gene assay is extremely sensitive and is especially suitable for quantifying dual luciferase expression in recombinant cells or in cell free transcription/translation reactions. Assays can be performed in tubes, cuvettes or multi-well plates. |
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Storage : | -20ºC |
Size : | 100 tests |
Products Types
◆ Recombinant Protein | ||
Luciferase-10R | Active Recombinant Renilla reniformis Luciferase (1-311), N-His-tagged | +Inquiry |
Luciferase-105G | Active Recombinant Gaussia Luciferase Protein, Streptavidin-Labled | +Inquiry |
Luciferase-10F | Recombinant Firefly Luciferase, Thermostable | +Inquiry |
Luciferase-168F | Recombinant Firefly Luciferase Protein | +Inquiry |
Luciferase-787F | Active Recombinant Firefly Luciferase Protein (1-550), N-His-tagged | +Inquiry |
◆ Native Protein | ||
Luciferase-10V | Native Vibrio fischeri Luciferase | +Inquiry |
Luciferase-09F | Active Native Firefly Luciferase | +Inquiry |
◆ Assay kits | ||
Kit-0527 | Luciferase Assay Kit | +Inquiry |
Kit-0529 | Renilla Luciferase Assay Kit | +Inquiry |
Kit-0357 | Gaussia Luciferase Receptor Gene Assay Kit | +Inquiry |
Kit-2421 | Luciferase Reporter Gene Assay Kit | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Customer Reviews (3)
Write a reviewWorked well, happy customer.
Great in lab experiments.
Satisfied with the purchase.
Q&As (7)
Ask a questionIt allows for real-time tracking of cellular and molecular processes in living organisms.
Luciferase enables bioluminescence, helping organisms attract mates, prey, or deter predators.
It catalyzes the oxidation of luciferin, releasing light.
Luciferase is found in fireflies, certain jellyfish, and some fungi and bacteria.
Yes, there are different types, varying in their substrate specificity and the color of emitted light.
ATP provides energy for the luciferase reaction, converting luciferin to oxyluciferin.
Luciferase provides a non-invasive and highly sensitive method for studying biological phenomena.
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