Dual Luciferase Reporter Gene Assay Kit

Cat.No. : Kit-2422
Product Overview : The accuracy of reporter assays can be improved by utilizing a dual reporter system. One of the reporter genes is correlated with the promoter of interest and is used to assess the effects of specific experimental conditions, while the second reporter is used as a control and serves as a baseline response. The Dual-Luciferase Reporter Gene Assay allows for the sequential measurement of the activity of two different luciferases, firefly (FFL) and Renilla (RL), in a single sample. The firefly luciferase luminescence is measured first by addition of the FFL Reagent. Next, the RL Reagent is added to the same well. The RL Reagent simultaneously quenches the firefly luciferase luminescence and initiates the Renilla luciferase reaction. The light production of both reactions can be conveniently measured on a luminometer. This bioluminescent dual reporter gene assay is extremely sensitive and is especially suitable for quantifying dual luciferase expression in recombinant cells or in cell free transcription/translation reactions. Assays can be performed in tubes, cuvettes or multi-well plates.

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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05/18/2022

    Worked well, happy customer.

    08/30/2021

      Great in lab experiments.

      09/04/2020

        Satisfied with the purchase.

        Q&As (7)

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        How is luciferase utilized in bioluminescence imaging? 05/09/2022

        It allows for real-time tracking of cellular and molecular processes in living organisms.

        What is the primary function of luciferase in organisms that produce it? 03/12/2022

        Luciferase enables bioluminescence, helping organisms attract mates, prey, or deter predators.

        How does luciferase produce light in a biochemical reaction? 12/14/2020

        It catalyzes the oxidation of luciferin, releasing light.

        In which organisms is luciferase commonly found? 11/28/2020

        Luciferase is found in fireflies, certain jellyfish, and some fungi and bacteria.

        Are there different types of luciferase, and how do they vary? 07/14/2020

        Yes, there are different types, varying in their substrate specificity and the color of emitted light.

        What is the role of ATP in the luciferase reaction? 01/28/2020

        ATP provides energy for the luciferase reaction, converting luciferin to oxyluciferin.

        What advantages does the use of luciferase offer in scientific research? 10/18/2019

        Luciferase provides a non-invasive and highly sensitive method for studying biological phenomena.

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