GMP Recombinant Human FN1 Protein FN1-154HG

GMP Recombinant Human FN1 Protein

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GMP Recombinant Human FN1 Protein

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Cat.No. : FN1-154HG
Product Overview : Recombinant Human NF1, Pro1270-Ser1546&Ala1721-Thr2016, was produced in E. coli using non-animal reagents in an animal-free facility under cGMP guidelines.
Description : Fibronectin1(FN1) is a secreted protein and contains 12 fibronectin type-I domains,fibronectin type-II domains and 16 fibronectin type-III domains.Recombinant human fibronectin fragment, is a protein of ~63 kDa containing a central cell-binding domain, a high affinity heparin-binding domain II,and CS1 site within the alternatively spliced III CS region of human fibronectin. Cells bind to a VLA-4 ligand, a CS-I site, and a VLA-5 ligand, a cell attachment domain, and virus vectors binds to a heparin binding domain II, which co-locates the cell and the virus vector on NovoNectin. This process enhances the density of both cells and vectors, and facilitates the gene transduction in the result.
Source : E. coli
Species : Human
Form : Lyophilized.
Bio-activity : Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells.
When 1×10E5 cells/well areadded to Fibronectin-coated plates (7-13 ng/mL and 100μL/well), approximately 50%-80% will adhere specifically after 30 minutes at 37°C.
AA Sequence : MPTDLRFTNI GPDTMRVTWA PPPSIDLTNF LVRYSPVKNE EDVAELSISP SDNAVVLTNL LPGTEYVVSV SSVYEQHEST PLRGRQKTGL DSPTGIDFSD ITANSFTVHW IAPRATITGY RIRHHPEHFS GRPREDRVPH SRNSITLTNL TPGTEYVVSI VALNGREESP LLIGQQSTVS DVPRDLEVVA ATPTSLLISW DAPAVTVRYY RITYGETGGN SPVQEFTVPG SKSTATISGL KPGVDYTITV YAVTGRGDSP ASSKPISINY RTEIDKPSMA IPAPTDLKFT QVTPTSLSAQ WTPPNVQLTG YRVRVTPKEK TGPMKEINLA PDSSSVVVSG LMVATKYEVS VYALKDTLTS RPAQGVVTTL ENVSPPRRAR VTDATETTIT ISWRTKTETI TGFQVDAVPA NGQTPIQRTI KPDVRSYTIT GLQPGTDYKI YLYTLNDNAR SSPVVIDAST AIDAPSNLRF LATTPNSLLV SWQPPRARIT GYIIKYEKPG SPPREVVPRP RPGVTEATIT GLEPGTEYTI YVIALKNNQK SEPLIGRKKT DELPQLVTLP HPNLHGPEIL DVPST
Residual Host Cell DNA Content : <10pg/mg
Residual Host Cell Protein Content : <1ug/mg
Endotoxin : <0.1EU/ug
Purity : Greater than 95% as determined by reducing SDS-PAGE.
Storage : Lyophilized and reconstituted protein should be stored at -80 centigrade. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Reconstitution : Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration >100μg/ml.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Quality Statement : Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.
Shipping : The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.
Gene Name : FN1 fibronectin 1 [ Homo sapiens ]
Official Symbol : FN1
Synonyms : FN1; fibronectin 1; fibronectin; CIG; cold insoluble globulin; FINC; GFND2; LETS; migration stimulating factor; MSF; cold-insoluble globulin; migration-stimulating factor; FN; FNZ; ED-B; GFND
Gene ID : 2335
mRNA Refseq : NM_002026
Protein Refseq : NP_002017
MIM : 135600
UniProt ID : P02751

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