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GMP Recombinant Human GDNF Protein

Cat.No.: GDNF-133HG
Product Overview: Recombinant Human GDNF, Arg109-Ile211, was produced in NS0 cell under cGMP guidelines.
Source: NS0
Species: Human
Form: Lyophilized from a 0.2 μm filtered solution in PBS.
Bio-activity: Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells.
The specific activity of recombinant human GDNF is approximately 3.1 x 10^3 units/μg, which is calibrated against recombinant human GDNF Reference Standard (NIBSC code: 09/266).
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human GFR alpha ‑1/GDNF R alpha ‑1 Fc Chimera at 1 µg/mL can bind Recombinant Human GDNF with an apparent Kd <1 nM.
Molecular Mass: Predicted Molecular Mass: 11.6 (monomer) kDa
N-terminal Sequence Analysis: Arg109-Gly-Gln-Arg-Gly-Lys-Asn-Arg-Gly-(Cys)
Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
Purity: >97%, by SDS-PAGE with silver staining, under reducing conditions.
Storage: Avoid repeated freeze-thaw cycles.
12 months, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution: Reconstitute at 100 μg/mL in PBS.
Quality Statement: Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.
Gene Name: GDNF glial cell derived neurotrophic factor [ Homo sapiens ]
Official Symbol: GDNF
Synonyms: GDNF; ATF1; ATF2; glial cell line derived neurotrophic factor; glial derived neurotrophic factor; HFB1 GDNF; ATF; astrocyte-derived trophic factor; HSCR3; HFB1-GDNF;
Gene ID: 2668
mRNA Refseq: NM_000514
Protein Refseq: NP_000505
MIM: 600837
UniProt ID: P39905

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