| Cat.No.: | IL11-101HG |
| Product Overview: | Recombinant Human IL11, Pro22-Leu199, was produced in Sf 9 (baculovirus)-derived in an animal component free process under cGMP guidelines. |
| Source: | Insect cell |
| Species: | Human |
| Form: | Lyophilized from a 0.2 μm filtered solution in PBS and EDTA. |
| Bio-activity: | Measured in a cell proliferation assay using T11 mouse plasmacytoma cells. Nordan, R.P. et al. (1987) J. Immunol. 139:813. The ED50 for this effect is 0.02-0.12 ng/mL. The specific activity of recombinant human IL-11 is approximately 1.05 x 10^4 units/μg, which is calibrated against recombinant human IL-11 WHO Standard (NIBSC code: 92/788). |
| Molecular Mass: | Predicted Molecular Mass: 19 kDa SDS-PAGE: 20 kDa, reducing conditions |
| N-terminal Sequence Analysis: | Pro-Gly-Pro-Pro-Pro-Gly-Pro-Pro-Arg-Val |
| Endotoxin: | <0.10 EU per 1 μg of the protein by the LAL method. |
| Purity: | >95%, by SDS-PAGE with silver staining, under reducing conditions. |
| Storage: | Avoid repeated freeze-thaw cycles. 12 months, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
| Reconstitution: | Reconstitute at 100 μg/mL in PBS. |
| Quality Statement: | Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing. |
| Shipping: | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
| Gene Name: | IL11 interleukin 11 [ Homo sapiens ] |
| Official Symbol: | IL11 |
| Synonyms: | IL11; interleukin 11; interleukin-11; adipogenesis inhibitory factor; AGIF; IL 11; oprelvekin; IL-11; |
| Gene ID: | 3589 |
| mRNA Refseq: | NM_000641 |
| Protein Refseq: | NP_000632 |
| MIM: | 147681 |
| UniProt ID: | P20809 |
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