|Product Overview:||Recombinant Human IL11, Pro22-Leu199, was produced in Sf 9 (baculovirus)-derived in an animal component free process under cGMP guidelines.|
|Form:||Lyophilized from a 0.2 μm filtered solution in PBS and EDTA.|
|Bio-activity:||Measured in a cell proliferation assay using T11 mouse plasmacytoma cells. Nordan, R.P. et al. (1987) J. Immunol. 139:813. The ED50 for this effect is 0.02-0.12 ng/mL.
The specific activity of recombinant human IL-11 is approximately 1.05 x 10^4 units/μg, which is calibrated against recombinant human IL-11 WHO Standard (NIBSC code: 92/788).
|Molecular Mass:||Predicted Molecular Mass: 19 kDa
SDS-PAGE: 20 kDa, reducing conditions
|N-terminal Sequence Analysis:||Pro-Gly-Pro-Pro-Pro-Gly-Pro-Pro-Arg-Val|
|Endotoxin:||<0.10 EU per 1 μg of the protein by the LAL method.|
|Purity:||>95%, by SDS-PAGE with silver staining, under reducing conditions.|
|Storage:||Avoid repeated freeze-thaw cycles.
12 months, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
|Reconstitution:||Reconstitute at 100 μg/mL in PBS.|
|Quality Statement:||Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.|
|Shipping:||The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.|
|Gene Name:||IL11 interleukin 11 [ Homo sapiens ]|
|Synonyms:||IL11; interleukin 11; interleukin-11; adipogenesis inhibitory factor; AGIF; IL 11; oprelvekin; IL-11;|