GMP Recombinant Human IL4 Protein
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|Product Overview :||Recombinant Human IL4 (His25-Ser153) was produced in HEK293 cell in an animal component free process under cGMP guidelines.|
|Description :||Interleukin-4 (IL-4) is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. IL-4 is produced by mast cells, T cells, and bone marrow stromal cells. IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergic response.|
|Source :||HEK293 cell|
|Bio-activity :||Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells.
The ED50 for this effect is typically 0.08-0.2 ng/ml.
|AA Sequence :||HKCDITLQEI IKTLNSLTEQ KTLCTELTVT DIFAASKNTT EKETFCRAAT VLRQFYSHHE KDTRCLGATA QQFHRHKQLI RFLKRLDRNL WGLAGLNSCP VKEANQSTLE NFLERLKTIM REKYSKCSS|
|Residual Host Cell DNA Content :||<10pg/mg|
|Residual Host Cell Protein Content :||<1ug/mg|
|Purity :||Greater than 95% as determined by reducing SDS-PAGE.|
|Usage :||Recommemnd working concentration: 50-100ng/ml or 500-1000U/ml|
|Storage :||Lyophilized and reconstituted protein should be stored at -80 centigrade. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.|
|Reconstitution :||Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration >100μg/ml.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
|Quality Statement :||Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.|
|Shipping :||The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.|
|Gene Name :||IL4 interleukin 4 [ Homo sapiens ]|
|Official Symbol :||IL4|
|Synonyms :||IL4; interleukin 4; interleukin-4; B cell growth factor 1; B_cell stimulatory factor 1; BCGF 1; BCGF1; BSF1; IL 4; lymphocyte stimulatory factor 1; binetrakin; pitrakinra; IL-4; BSF-1; BCGF-1|
|Gene ID :||3565|
|mRNA Refseq :||NM_000589|
|Protein Refseq :||NP_000580|
|UniProt ID :||P05112|
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