|Product Overview:||Recombinant Human INHBA, Gly311-Ser426, was produced in CHO cell under cGMP guidelines.|
|Form:||Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.|
|Bio-activity:||Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. Schwall, R.H. et al. (1991) Method Enzymol. 198:340. The ED50 for this effect is 0.2-1.2 ng/mL.
The specific activity of Recombinant Human/Mouse/Rat Activin A is approximately 1 IU/μg, which is calibrated against human Activin A WHO International Standard (NIBSC code: 91/626).
|Molecular Mass:||Predicted Molecular Mass: 13 (monomer) kDa
SDS-PAGE: 14 kDa, reducing conditions
24 kDa, non-reducing conditions
|N-terminal Sequence Analysis:||Gly311-Leu-Glu-(Cys)-Asp-Gly-Lys-Val-Asn-Ile|
|Endotoxin:||<0.10 EU per 1 μg of the protein by the LAL method.|
|Purity:||>97%, by SDS-PAGE with silver staining.|
|Storage:||Avoid repeated freeze-thaw cycles.
12 months, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
|Reconstitution:||Reconstitute at 100-500 μg/mL in sterile 4 mM HCl.|
|Quality Statement:||Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.|
|Shipping:||The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.|
|Gene Name:||INHBA inhibin, beta A [ Homo sapiens ]|
|Synonyms:||INHBA; inhibin, beta A;inhibin beta A chain; Inhibin, beta-1; activin beta-A chain; EDF; FRP;|