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| Cat.No.: | INHBA-114HG |
| Product Overview: | Recombinant Human INHBA, Gly311-Ser426, was produced in CHO cell under cGMP guidelines. |
| Source: | CHO cell |
| Species: | Human |
| Form: | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
| Bio-activity: | Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. Schwall, R.H. et al. (1991) Method Enzymol. 198:340. The ED50 for this effect is 0.2-1.2 ng/mL. The specific activity of Recombinant Human/Mouse/Rat Activin A is approximately 1 IU/μg, which is calibrated against human Activin A WHO International Standard (NIBSC code: 91/626). |
| Molecular Mass: | Predicted Molecular Mass: 13 (monomer) kDa SDS-PAGE: 14 kDa, reducing conditions 24 kDa, non-reducing conditions |
| N-terminal Sequence Analysis: | Gly311-Leu-Glu-(Cys)-Asp-Gly-Lys-Val-Asn-Ile |
| Endotoxin: | <0.10 EU per 1 μg of the protein by the LAL method. |
| Purity: | >97%, by SDS-PAGE with silver staining. |
| Storage: | Avoid repeated freeze-thaw cycles. 12 months, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
| Reconstitution: | Reconstitute at 100-500 μg/mL in sterile 4 mM HCl. |
| Quality Statement: | Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing. |
| Shipping: | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
| Gene Name: | INHBA inhibin, beta A [ Homo sapiens ] |
| Official Symbol: | INHBA |
| Synonyms: | INHBA; inhibin, beta A;inhibin beta A chain; Inhibin, beta-1; activin beta-A chain; EDF; FRP; |
| Gene ID: | 3624 |
| mRNA Refseq: | NM_002192 |
| Protein Refseq: | NP_002183 |
| MIM: | 147290 |
| UniProt ID: | P08476 |
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