|Product Overview:||Recombinant Human NTF3, Tyr139-Thr257, was produced in Sf 21 (baculovirus)-derived under cGMP guidelines.|
|Form:||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Bio-activity:||Measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB. The ED50 for this effect is 1-10 ng/mL.
The specific activity of Recombinant Human NT-3 is approximately 2.1 x 10^3 units/μg, which is calibrated against recombinant human NT-3 Standard (NIBSC code: 98/718).
|Molecular Mass:||Predicted Molecular Mass: 13.7 (monomer) kDa|
|N-terminal Sequence Analysis:||Tyr-Ala-Glu-His-Lys-Ser-His-Arg-Gly-Glu|
|Endotoxin:||<0.10 EU per 1 μg of the protein by the LAL method.|
|Purity:||>97%, by SDS-PAGE with silver staining.|
|Storage:||Avoid repeated freeze-thaw cycles.
12 months, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
|Reconstitution:||Reconstitute at 100 μg/mL in PBS.|
|Quality Statement:||Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.|
|Shipping:||The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.|
|Gene Name:||NTF3 neurotrophin 3 [ Homo sapiens ]|
|Synonyms:||NTF3; neurotrophin 3; neurotrophin-3; NGF2; NT-3; NT3; HDNF; NGF-2|