Human Adipose Tissue Lysate

Cat.No. : Adipose-1H
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Species : Human
Form : The human adipose tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors.
Concentration : 3.0 mg/ml
Tissue Type : Adipose
Preparation method : Human adipose tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human adipose tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the adipose tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The adipose tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each.
Safety Testing : This material has been tested by accepted techniques and has been found to be negative for HBsAg, HIV 1/2, and HCV. Additional patient information may be available upon request.
Diagnosis : Normal
Expiration Date : Six months from date of receipt
Size : 0.1 mg, 0.5 mg, 1.0 mg
Storage Instruction : This lysate should be aliquoted in working amounts and may be stored for up to one month at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. For longer term storage, freeze working aliquots at -70°C.
Applications : This lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (13)

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What are adipose proteins? 04/12/2022

Adipose proteins are proteins that are secreted by adipose (fat) tissue, including adipocytes and stromal cells. These proteins have diverse roles in metabolic regulation, inflammation, and other physiological processes.

How is adipose tissue involved in the regulation of appetite and energy expenditure? 11/10/2020

Adipose tissue secretes the hormone leptin, which acts in the hypothalamus to reduce appetite and increase energy expenditure. Leptin released from adipocytes reflects the amount of body fat, so a decrease in body fat leads to a decrease in leptin secretion, which, in turn, stimulates appetite and reduces energy expenditure. Additionally, adipose tissue also secretes hormones such as ghrelin, insulin, and glucagon-like peptide-1 (GLP-1), which can affect appetite and energy balance.

How can adipose proteins be targeted for medical treatment? 08/20/2020

Adipose proteins can be targeted for medical treatment through the development of drugs that specifically interact with and modulate their activity. For example, adiponectin agonists or leptin antagonists could potentially be used as treatments for obesity and associated metabolic disorders.

How is adiponectin regulated in the body? 11/04/2019

Adiponectin secretion is regulated by various factors such as obesity, insulin resistance, and inflammation. Adiponectin levels are typically decreased in obesity and associated metabolic disorders.

How does the amount of adipose tissue affect the secretion of adipose proteins? 07/17/2019

Adipose tissue mass is positively correlated with adipose protein secretion. That is, the more adipose tissue in the body, the more adipokines and other adipose proteins are secreted into the bloodstream.

How can adipose tissue be used in regenerative medicine? 07/15/2019

Adipose tissue is a rich source of stem cells, which have the potential to differentiate into various cell types such as adipocytes, osteoblasts, and chondrocytes. These stem cells can be used in regenerative medicine to repair or replace damaged tissues or organs.

Are there any current medications that target adipose proteins? 11/11/2018

There are currently no FDA-approved medications that specifically target adipose proteins. However, a number of investigational drugs are in development for this purpose.

What are some medical conditions associated with dysfunction of adipose proteins? 11/08/2018

Diseases and conditions associated with dysfunction of adipose proteins include obesity, type 2 diabetes, metabolic syndrome, cardiovascular disease, and various inflammatory disorders.

Can the regulation of adipose protein secretion be a potential target for the treatment of metabolic diseases? 12/28/2017

Yes, adipose protein secretion can be a potential target for the treatment of metabolic diseases such as obesity and type 2 diabetes. Targeting the secretion of adipose proteins could help regulate inflammation, insulin resistance, and other metabolic processes that contribute to the pathogenesis of these diseases. Drugs targeting the secretion or activity of specific adipokines, such as adiponectin, have been already developed or are in development.

Are there any lifestyle modifications that can affect the secretion of adipose proteins? 06/30/2017

Yes, lifestyle modifications such as weight loss, exercise, and diet can affect the secretion of adipose proteins. For example, weight loss is associated with increased adiponectin levels, while exercise has been shown to increase the secretion of anti-inflammatory adipokines.

Are there any genetic factors that influence adipose protein levels? 07/03/2016

Yes, genetic factors can influence adipose protein levels, leading to variation in susceptibility to metabolic disorders such as obesity and diabetes. For example, variations in the genes that regulate adiponectin secretion have been associated with increased risk of metabolic diseases.

Can adipose tissue inflammation be reversed? 05/30/2016

Yes, adipose tissue inflammation can be reversed with lifestyle modifications such as weight loss, exercise, and diet changes. These interventions can reduce adipose tissue mass, decrease the production of pro-inflammatory cytokines, and increase the secretion of anti-inflammatory adipokines and other factors. Medications that target inflammation can also decrease adipose tissue inflammation.

How can studying adipose proteins contribute to medical advances in the future? 03/09/2016

Studying adipose proteins can lead to a better understanding of the mechanisms underlying metabolic and inflammatory disorders, and identify potential targets for therapeutic intervention. This could ultimately lead to the development of more effective treatments for a range of diseases and conditions.

Customer Reviews (4)

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Reviews
01/04/2022

    The protein is very pure and stable in the experiment.

    10/15/2019

      the manufacturer's remarkable technical support is exceptional and can easily and promptly solve any issues I encounter.

      05/08/2017

        The purity of this protein is exceptional, making it perfect for meeting the specific requirements of my experiments.

        03/14/2017

          I am highly satisfied with this product and the company's service and am confident in using it for my research.

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