Human DDB2 Knockdown Cell Lysate

• Cat.No.: DDB2-501HKCL
• Product Overview: WB-validated DDB2 Knockdown HT-1080 Cell Lysate

Specification

• Species: Human
• Source: HT-1080
• Tag: Non
• Description: This gene encodes a protein that is necessary for the repair of ultraviolet light-damaged DNA. This protein is the smaller subunit of a heterodimeric protein complex that participates in nucleotide excision repair, and this complex mediates the ubiquitylation of histones H3 and H4, which facilitates the cellular response to DNA damage. This subunit appears to be required for DNA binding. Mutations in this gene cause xeroderma pigmentosum complementation group E, a recessive disease that is characterized by an increased sensitivity to UV light and a high predisposition for skin cancer development, in some cases accompanied by neurological abnormalities. Two transcript variants encoding different isoforms have been found for this gene.
• Form: Cell-Tissue Lysis buffer
• Molecular Mass: 48 kDa
• Notes: Instruction of use: This knockdown cell lysate should be paired with wild-type HT-1080 cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins.
• Storage: Store at -20 centigrade for two years.
• Concentration: Lot-specific
• Shipping: Blue Ice
• Components: 1 vial of 100 μg WT HT-1080 cell lysate 1 vial of 100 μg DDB2 KD HT-1080 cell lysate
• Protein Families: Druggable Genome
• Protein Pathways: Nucleotide excision repair, p53 signaling pathway, Ubiquitin mediated proteolysis
• Lysate QC: RT-qPCR; Western Blotting (WB)

Gene Information

• Gene Name: DDB2 damage-specific DNA binding protein 2, 48kDa [ Homo sapiens (human) ]
• Official Symbol: DDB2
• Synonyms: DDB2; damage-specific DNA binding protein 2, 48kDa; damage specific DNA binding protein 2 (48kD); DNA damage-binding protein 2; DDB p48 subunit; DDBB; FLJ34321; UV damaged DNA binding protein 2; UV DDB2; xeroderma pigmentosum group E protein; UV-DDB 2; UV-damaged DNA-binding protein 2; damage-specific DNA-binding protein 2; UV-DDB2
• Gene ID: 1643
• mRNA Refseq: NM_000107
• Protein Refseq: NP_000098
• MIM: 600811
• UniProt ID: Q92466