Human DGCR8 Knockdown Cell Lysate

• Cat.No.: DGCR8-337HKCL
• Product Overview: WB-validated DGCR8 Knockdown HeLa Cell Lysate

Specification

• Species: Human
• Source: HeLa
• Tag: Non
• Description: This gene encodes a subunit of the microprocessor complex which mediates the biogenesis of microRNAs from the primary microRNA transcript. The encoded protein is a double-stranded RNA binding protein that functions as the non-catalytic subunit of the microprocessor complex. This protein is required for binding the double-stranded RNA substrate and facilitates cleavage of the RNA by the ribonuclease III protein, Drosha. Alternate splicing results in multiple transcript variants.
• Form: Cell-Tissue Lysis buffer
• Molecular Mass: 86 kDa
• Notes: Instruction of use: This knockdown cell lysate should be paired with wild-type HeLa cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins.
• Storage: Store at -20 centigrade for two years.
• Concentration: Lot-specific
• Shipping: Blue Ice
• Components: 1 vial of 100 μg WT HeLa cell lysate 1 vial of 100 μg DGCR8 KD HeLa cell lysate
• Lysate QC: RT-qPCR; Western Blotting (WB)

Gene Information

• Gene Name: DGCR8 DiGeorge syndrome critical region gene 8 [ Homo sapiens (human) ]
• Official Symbol: DGCR8
• Synonyms: DGCR8; DiGeorge syndrome critical region gene 8; C22orf12, chromosome 22 open reading frame 12; microprocessor complex subunit DGCR8; DGCRK6; Gy1; pasha; DiGeorge syndrome critical region 8; C22orf12
• Gene ID: 54487
• mRNA Refseq: NM_001190326
• Protein Refseq: NP_001177255
• MIM: 609030
• UniProt ID: Q8WYQ5