Recombinant Human DGCR8 Protein, GST-tagged

• Cat.No.: DGCR8-2567H
• Product Overview: Human DGCR8 full-length ORF ( NP_073557.3, 1 a.a. - 773 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: This gene encodes a subunit of the microprocessor complex which mediates the biogenesis of microRNAs from the primary microRNA transcript. The encoded protein is a double-stranded RNA binding protein that functions as the non-catalytic subunit of the microprocessor complex. This protein is required for binding the double-stranded RNA substrate and facilitates cleavage of the RNA by the ribonuclease III protein, Drosha. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 112.4 kDa
• AA Sequence: METDESPSPLPCGPAGEAVMESRAR PFQALPREQSPPPPLQTSSGAEVMD VGSGGDGQSELPAEDPFNFYGASLL SKGSFSKGRLLIDPNCSGHSPRTAR HAPAVRKFSPDLKLLKDVKISVSFT ESCRSKDRKVLYTGAERDVRAECGL LLSPVSGDVHACPFGGSVGDGVGIG GESADKKDEENELDQEKRVEYAVLD ELEDFTDNLELDEEGAGGFTAKAIV QRDRVDEEALNFPYEDDFDNDVDAL LEEGLCAPKKRRTEEKYGGDSDHPS DGETSVQPMMTKIKTVLKSRGRPPT EPLPDGWIMTFHNSGVPVYLHRESR VVTWSRPYFLGTGSIRKHDPPLSSI PCLHYKKMKDNEEREQSSDLTPSGD VSPVKPLSRSAELEFPLDEPDSMGA DPGPPDEKDPLGAEAAPGALGQVKA KVEVCKDESVDLEEFRSYLEKRFDF EQVTVKKFRTWAERRQFNREMKRKQ AESERPILPANQKLITLSVQDAPTK KEFVINPNGKSEVCILHEYMQRVLK VRPVYNFFECENPSEPFGASVTIDG VTYGSGTASSKKLAKNKAARATLEI LIPDFVKQTSEEKPKDSEELEYFNH ISIEDSRVYELTSKAGLLSPYQILH ECLKRNHGMGDTSIKFEVVPGKNQK SEYVMACGKHTVRGWCKNKRVGKQL ASQKILQLLHPHVKNWGSLLRMYGR ESSKMVKQETSDKSVIELQQYAKKN KPNLHILSKLQEEMKRLAEEREETR KKPKMSIVASAQPGGEPLCTVDV
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: DGCR8 DiGeorge syndrome critical region gene 8 [ Homo sapiens ]
• Official Symbol: DGCR8
• Synonyms: DGCR8; DiGeorge syndrome critical region gene 8; C22orf12, chromosome 22 open reading frame 12; microprocessor complex subunit DGCR8; DGCRK6; Gy1; pasha; DiGeorge syndrome critical region 8; C22orf12
• Gene ID: 54487
• mRNA Refseq: NM_001190326
• Protein Refseq: NP_001177255
• MIM: 609030
• UniProt ID: Q8WYQ5