Recombinant Full Length Human DLD Protein, C-Flag-tagged

• Cat.No.: DLD-1358HFL
• Product Overview: Recombinant Full Length Human DLD Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, the encoded protein functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, this protein can function as a protease. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. Alternative splicing results in multiple transcript variants.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 50.1 kDa
• AA Sequence: MQSWSRVYCSLAKRGHFNRISHGLQ GLSAVPLRTYADQPIDADVTVIGSG PGGYVAAIKAAQLGFKTVCI EKNETLGGTCLNVGCIPSKALLNNS HYYHMAHGKDFASRGIEMSEVRLNL DKMMEQKSTAVKALTGGIAH LFKQNKVVHVNGYGKITGKNQVTAT KADGGTQVIDTKNILIATGSEVTPF PGITIDEDTIVSSTGALSLK KVPEKMVVIGAGVIGVELGSVWQRL GADVTAVEFLGHVGGVGIDMEISKN FQRILQKQGFKFKLNTKVTG ATKKSDGKIDVSIEAASGGKAEVIT CDVLLVCIGRRPFTKNLGLEELGIE LDPRGRIPVNTRFQTKIPNI YAIGDVVAGPMLAHKAEDEGIICVE GMAGGAVHIDYNCVPSVIYTHPEVA WVGKSEEQLKEEGIEYKVGK FPFAANSRAKTNADTDGMVKILGQK STDRVLGAHILGPGAGEMVNEAALA LEYGASCEDIARVCHAHPTL
  SEAFREANLAASFGKSINFTRTRPL EQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Citrate cycle (TCA cycle), Glycine, serine and threonine metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Pyruvate metabolism, Valine, leucine and isoleucine degradation

Gene Information

• Gene Name: DLD dihydrolipoamide dehydrogenase [ Homo sapiens (human) ]
• Official Symbol: DLD
• Synonyms: E3; LAD; DLDD; DLDH; GCSL; PHE3; OGDC-E3
• Gene ID: 1738
• mRNA Refseq: NM_000108.5
• Protein Refseq: NP_000099.2
• MIM: 238331
• UniProt ID: P09622