| Species : |
Human |
| Source : |
HeLa |
| Tag : |
Non |
| Description : |
Dynamins represent one of the subfamilies of GTP-binding proteins. These proteins share considerable sequence similarity over the N-terminal portion of the molecule, which contains the GTPase domain. Dynamins are associated with microtubules. They have been implicated in cell processes such as endocytosis and cell motility, and in alterations of the membrane that accompany certain activities such as bone resorption by osteoclasts. Dynamins bind many proteins that bind actin and other cytoskeletal proteins. Dynamins can also self-assemble, a process that stimulates GTPase activity. Five alternatively spliced transcripts encoding different proteins have been described. Additional alternatively spliced transcripts may exist, but their full-length nature has not been determined. |
| Form : |
Cell-Tissue Lysis buffer |
| Molecular Mass : |
98 kDa |
| Notes : |
Instruction of use: This knockdown cell lysate should be paired with wild-type HeLa cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins. |
| Storage : |
Store at -20 centigrade for two years. |
| Concentration : |
Lot-specific |
| Shipping : |
Blue Ice |
| Components : |
1 vial of 100 μg WT HeLa cell lysate
1 vial of 100 μg DNM2 KD HeLa cell lysate |
| Protein Families : |
Transcription Factors |
| Protein Pathways : |
Endocytosis, Fc gamma R-mediated phagocytosis |
| Lysate QC : |
RT-qPCR; Western Blotting (WB) |
