Human DPYD Knockdown Cell Lysate

• Cat.No.: DPYD-142HKCL
• Product Overview: WB-validated DPYD Knockdown HeLa Cell Lysate

Specification

• Species: Human
• Source: HeLa
• Tag: Non
• Description: The protein encoded by this gene is a pyrimidine catabolic enzyme and the initial and rate-limiting factor in the pathway of uracil and thymidine catabolism. Mutations in this gene result in dihydropyrimidine dehydrogenase deficiency, an error in pyrimidine metabolism associated with thymine-uraciluria and an increased risk of toxicity in cancer patients receiving 5-fluorouracil chemotherapy. Two transcript variants encoding different isoforms have been found for this gene.
• Form: Cell-Tissue Lysis buffer
• Molecular Mass: 111 kDa
• Notes: Instruction of use: This knockdown cell lysate should be paired with wild-type HeLa cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins.
• Storage: Store at -20 centigrade for two years.
• Concentration: Lot-specific
• Shipping: Blue Ice
• Components: 1 vial of 100 μg WT HeLa cell lysate 1 vial of 100 μg DPYD KD HeLa cell lysate
• Protein Families: Druggable Genome
• Protein Pathways: beta-Alanine metabolism, Drug metabolism - other enzymes, Metabolic pathways, Pantothenate and CoA biosynthesis, Pyrimidine metabolism
• Lysate QC: RT-qPCR; Western Blotting (WB)

Gene Information

• Gene Name: DPYD dihydropyrimidine dehydrogenase [ Homo sapiens (human) ]
• Official Symbol: DPYD
• Synonyms: DPYD; dihydropyrimidine dehydrogenase; dihydropyrimidine dehydrogenase [NADP(+)]; DPD; dihydrouracil dehydrogenase; dihydrothymine dehydrogenase; DHP; DHPDHASE; MGC70799; MGC132008
• Gene ID: 1806
• mRNA Refseq: NM_000110
• Protein Refseq: NP_000101
• MIM: 612779
• UniProt ID: Q12882