Human DROSHA Knockdown Cell Lysate

• Cat.No.: DROSHA-143HKCL
• Product Overview: WB-validated DROSHA Knockdown HeLa Cell Lysate

Specification

• Species: Human
• Source: HeLa
• Tag: Non
• Description: This gene encodes a ribonuclease (RNase) III double-stranded RNA-specific ribonuclease and subunit of the microprocessor protein complex, which catalyzes the initial processing step of microRNA (miRNA) synthesis. The encoded protein cleaves the stem loop structure from the primary microRNA (pri-miRNA) in the nucleus, yielding the precursor miRNA (pre-miRNA), which is then exported to the cytoplasm for further processing. In a human cell line lacking a functional copy of this gene, canonical miRNA synthesis is reduced. Somatic mutations in this gene have been observed in human patients with kidney cancer.
• Form: Cell-Tissue Lysis buffer
• Molecular Mass: 159 kDa
• Notes: Instruction of use: This knockdown cell lysate should be paired with wild-type HeLa cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins.
• Storage: Store at -20 centigrade for two years.
• Concentration: Lot-specific
• Shipping: Blue Ice
• Components: 1 vial of 100 μg WT HeLa cell lysate 1 vial of 100 μg DROSHA KD HeLa cell lysate
• Lysate QC: RT-qPCR; Western Blotting (WB)

Gene Information

• Gene Name: DROSHA drosha, ribonuclease type III [ Homo sapiens (human) ]
• Official Symbol: DROSHA
• Synonyms: DROSHA; drosha, ribonuclease type III; ribonuclease type III, nuclear, RNASEN; ribonuclease 3; drosha; double stranded RNA specific endoribonuclease; ribonuclease type III; Etohi2; HSA242976; RN3; RNASE3L; p241; RNase III; protein Drosha; nuclear RNase III Drosha; putative ribonuclease III; ribonuclease III, nuclear; ribonuclease type III, nuclear; drosha, double-stranded RNA-specific endoribonuclease; putative protein p241 which interacts with transcription factor Sp1; ETOHI2; RNASEN; RANSE3L
• Gene ID: 29102
• mRNA Refseq: NM_001100412
• Protein Refseq: NP_001093882
• MIM: 608828
• UniProt ID: Q9NRR4