Human EIF4EBP1 Knockdown Cell Lysate

• Cat.No.: EIF4EBP1-153HKCL
• Product Overview: WB-validated EIF4EBP1 Knockdown HeLa Cell Lysate

Specification

• Species: Human
• Source: HeLa
• Tag: Non
• Description: This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
• Form: Cell-Tissue Lysis buffer
• Molecular Mass: 13 kDa
• Notes: Instruction of use: This knockdown cell lysate should be paired with wild-type HeLa cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins.
• Storage: Store at -20 centigrade.
• Concentration: Lot-specific
• Shipping: Blue Ice
• Components: 1 vial of 100 μg WT HeLa cell lysate 1 vial of 100 μg EIF4EBP1 KD HeLa cell lysate
• Protein Pathways: Acute myeloid leukemia, ErbB signaling pathway, Insulin signaling pathway, mTOR signaling pathway
• Lysate QC: RT-qPCR; Western Blotting (WB)

Gene Information

• Gene Name: EIF4EBP1 eukaryotic translation initiation factor 4E binding protein 1 [ Homo sapiens (human) ]
• Official Symbol: EIF4EBP1
• Synonyms: EIF4EBP1; eukaryotic translation initiation factor 4E binding protein 1; eukaryotic translation initiation factor 4E-binding protein 1; 4E BP1; PHAS I; phosphorylated heat and acid stable protein regulated by insulin 1; eIF4E-binding protein 1; phosphorylated heat- and acid-stable protein regulated by insulin 1; BP-1; 4EBP1; 4E-BP1; PHAS-I; MGC4316
• Gene ID: 1978
• mRNA Refseq: NM_004095
• Protein Refseq: NP_004086
• MIM: 602223
• UniProt ID: Q13541