Human EOGT Knockdown Cell Lysate

• Cat.No.: EOGT-482HKCL
• Product Overview: WB-validated EOGT Knockdown HT-1080 Cell Lysate

Specification

• Species: Human
• Source: HT-1080
• Tag: Non
• Description: This gene encodes an enzyme that acts in the lumen of the endoplasmic reticulum to catalyze the transfer of N-acetylglucosamine to serine or threonine residues of extracellular-targeted proteins. This enzyme modifies proteins containing eukaryotic growth factor (EGF)-like domains, including the Notch receptor, thereby regulating developmental signalling. Mutations in this gene have been observed in individuals with Adams-Oliver syndrome 4. Alternative splicing results in multiple transcript variants.
• Form: Cell-Tissue Lysis buffer
• Molecular Mass: 62 kDa
• Notes: Instruction of use: This knockdown cell lysate should be paired with wild-type HT-1080 cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins.
• Storage: Store at -20 centigrade for two years.
• Concentration: Lot-specific
• Shipping: Blue Ice
• Components: 1 vial of 100 μg WT HT-1080 cell lysate 1 vial of 100 μg EOGT KD HT-1080 cell lysate
• Lysate QC: RT-qPCR; Western Blotting (WB)

Gene Information

• Gene Name: EOGT EGF domain specific O-linked N-acetylglucosamine transferase [ Homo sapiens (human) ]
• Official Symbol: EOGT
• Synonyms: EOGT; EGF domain specific O-linked N-acetylglucosamine transferase; AOS4; AER61; EOGT1; C3orf64; EGF domain-specific O-linked N-acetylglucosamine transferase; AER61 glycosyltransferase; EGF domain-specific O-linked N-acetylglucosamine (GlcNAc) transferase; EGF-O-GlcNAc transferase; extracellular O-linked N-acetylglucosamine transferase; EC 2.4.1.255
• Gene ID: 285203
• mRNA Refseq: NM_001278689
• Protein Refseq: NP_001265618
• MIM: 614789
• UniProt ID: Q5NDL2