Human MADD Knockdown Cell Lysate

• Cat.No.: MADD-367HKCL
• Product Overview: WB-validated MADD Knockdown HeLa Cell Lysate

Specification

• Species: Human
• Source: HeLa
• Tag: Non
• Description: Tumor necrosis factor alpha (TNF-alpha) is a signaling molecule that interacts with one of two receptors on cells targeted for apoptosis. The apoptotic signal is transduced inside these cells by cytoplasmic adaptor proteins. The protein encoded by this gene is a death domain-containing adaptor protein that interacts with the death domain of TNF-alpha receptor 1 to activate mitogen-activated protein kinase (MAPK) and propagate the apoptotic signal. It is membrane-bound and expressed at a higher level in neoplastic cells than in normal cells. Several transcript variants encoding different isoforms have been described for this gene.
• Form: Cell-Tissue Lysis buffer
• Molecular Mass: 183 kDa
• Notes: Instruction of use: This knockdown cell lysate should be paired with wild-type HeLa cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins.
• Storage: Store at -20 centigrade for two years.
• Concentration: Lot-specific
• Shipping: Blue Ice
• Components: 1 vial of 100 μg WT HeLa cell lysate 1 vial of 100 μg MADD KD HeLa cell lysate
• Protein Families: Druggable Genome
• Lysate QC: RT-qPCR; Western Blotting (WB)

Gene Information

• Gene Name: MADD MAP-kinase activating death domain [ Homo sapiens (human) ]
• Official Symbol: MADD
• Synonyms: MADD; MAP-kinase activating death domain; MAP kinase-activating death domain protein; DENN; KIAA0358; RAB3GEP; Rab3 GDP/GTP exchange factor; insulinoma glucagonoma clone 20; differentially expressed in normal and neoplastic cells; IG20; FLJ35600; FLJ36300
• Gene ID: 8567
• mRNA Refseq: NM_001135943
• Protein Refseq: NP_001129415
• MIM: 603584
• UniProt ID: Q8WXG6