Recombinant Rat Madd Protein, His-tagged

• Cat.No.: Madd-1755R
• Product Overview: Recombinant Rat Madd Protein (Arg1291-Ser1602) with N-His tag was expressed in E. coli.

Specification

• Description: Enables guanyl-nucleotide exchange factor activity. Involved in negative regulation of growth hormone secretion and negative regulation of pancreatic amylase secretion. Located in cytosol; nucleus; and synaptic vesicle. Is active in synapse. Biomarker of transient cerebral ischemia. Orthologous to human MADD (MAP kinase activating death domain).
• Source: E. coli
• Species: Rat
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 39.8 kDa
  Accurate Molecular Mass: 40 kDa
• Protein length: Arg1291-Ser1602
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: Madd MAP-kinase activating death domain [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Madd
• Synonyms: MADD; MAP-kinase activating death domain; MAP kinase-activating death domain protein; rab3 GDP/GTP exchange factor; Rab3 GDP/GTP exchange protein
• Gene ID: 94193
• mRNA Refseq: NM_053585
• Protein Refseq: NP_446037
• UniProt ID: O08873