Recombinant Human MCM6 protein, His-tagged

• Cat.No.: MCM6-3398H
• Product Overview: Recombinant Human MCM6 protein(471-821 aa), fused with N-terminal His tag, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 471-821 aa
• Form: The purified protein was lyophilized from sterile phosphate-buffered saline (PBS: 58 mM Na₂HPO₄, 17 mM NaH₂PO₄, 68 mM NaCl, pH 7.4). Prior to lyophilization, 5% (w/v) trehalose and 5% (w/v) mannitol were incorporated as cryoprotective excipients. The protein was eluted with a buffer containing 300 mM imidazole.
• AASequence: VAIHEAMEQQTISITKAGVKATLNARTSILAAANPISGHYDRSKSLKQNINLSAPIMSRFDLFFILVDECNEVTDYAIARRIVDLHSRIEESIDRVYSLDDIRRYLLFARQFKPKISKESEDFIVEQYKHLRQRDGSGVTKSSWRITVRQLESMIRLSEAMARMHCCDEVQPKHVKEAFRLLNKSIIRVETPDVNLDQEEEIQMEVDEGAGGINGHADSPAPVNGINGYNEDINQESAPKASLRLGFSEYCRISNLIVLHLRKVEEEEDESALKRSELVNWYLKEIESEIDSEEELINKKRIIEKVIHRLTHYDHVLIELTQAGLKGSTEGSESYEEDPYLVVNPNYLLED
• Purity: 85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Store at 2-8°C for 1-2 weeks. Aliquot and store at -20°C to -80°C for up to 12 months, reconstitution with sterile water and addition of an equal volume of glycerol. Avoid repeat freeze-thaw cycles.
• Reconstitution: Dissolve the product in 200 μl sterile water to achieve a working concentration of 0.25 µg/μl. If experimental compatibility allows, mix the aqueous solution with an equal volume of glycerol (1:1 ratio) after initial reconstitution.

Gene Information

• Gene Name: MCM6 minichromosome maintenance complex component 6 [ Homo sapiens ]
• Official Symbol: MCM6
• Synonyms: MCM6; minichromosome maintenance complex component 6; MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe) (S. cerevisiae) , minichromosome maintenance deficient (mis5, S. pombe) 6 , minichromosome maintenance deficient 6 homolog (S. cerevisiae); DNA replication licensing factor MCM6; Mis5; MIS5 homolog (S.pombe); MIS5 homolog; minichromosome maintenance deficient 6 homolog; minichromosome maintenance deficient (mis5, S. pombe) 6; MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe); P105MCM; MCG40308
• Gene ID: 4175
• mRNA Refseq: NM_005915
• Protein Refseq: NP_005906
• MIM: 601806
• UniProt ID: Q14566