Human MCM7 Knockdown Cell Lysate

• Cat.No.: MCM7-239HKCL
• Product Overview: WB-validated MCM7 Knockdown HeLa Cell Lysate

Specification

• Species: Human
• Source: HeLa
• Tag: Non
• Description: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
• Form: Cell-Tissue Lysis buffer
• Molecular Mass: 81 kDa
• Notes: Instruction of use: This knockdown cell lysate should be paired with wild-type HeLa cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins.
• Storage: Store at -20 centigrade for two years.
• Concentration: Lot-specific
• Shipping: Blue Ice
• Components: 1 vial of 100 μg WT HeLa cell lysate 1 vial of 100 μg MCM7 KD HeLa cell lysate
• Protein Families: Transcription Factors
• Protein Pathways: Cell cycle, DNA replication
• Lysate QC: RT-qPCR; Western Blotting (WB)

Gene Information

• Gene Name: MCM7 minichromosome maintenance complex component 7 [ Homo sapiens (human) ]
• Official Symbol: MCM7
• Synonyms: MCM7; minichromosome maintenance complex component 7; MCM2, MCM7 minichromosome maintenance deficient 7 (S. cerevisiae), minichromosome maintenance deficient (S. cerevisiae) 7; DNA replication licensing factor MCM7; CDC47; CDC47 homolog; homolog of S. cerevisiae Cdc47; minichromosome maintenance deficient 7; MCM2; P85MCM; P1CDC47; PNAS146; P1.1-MCM3
• Gene ID: 4176
• mRNA Refseq: NM_005916
• Protein Refseq: NP_005907
• MIM: 600592
• UniProt ID: P33993