Human NLK Knockdown Cell Lysate

• Cat.No.: NLK-505HKCL
• Product Overview: WB-validated NLK Knockdown HT-1080 Cell Lysate

Specification

• Species: Human
• Source: HT-1080
• Tag: Non
• Description: Enables DNA-binding transcription factor binding activity; protein serine/threonine kinase activity; and ubiquitin protein ligase binding activity. Involved in several processes, including negative regulation of TORC1 signaling; protein stabilization; and transforming growth factor beta receptor signaling pathway. Predicted to be located in cytosol and nucleoplasm. Predicted to be active in cytoplasm and nucleus.
• Form: Cell-Tissue Lysis buffer
• Molecular Mass: 58 kDa
• Notes: Instruction of use: This knockdown cell lysate should be paired with wild-type HT-1080 cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins.
• Storage: Store at -20 centigrade for two years.
• Concentration: Lot-specific
• Shipping: Blue Ice
• Components: 1 vial of 100 μg WT HT-1080 cell lysate 1 vial of 100 μg NLK KD HT-1080 cell lysate
• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors
• Protein Pathways: Adherens junction, MAPK signaling pathway, Wnt signaling pathway
• Lysate QC: RT-qPCR; Western Blotting (WB)

Gene Information

• Gene Name: NLK nemo-like kinase [ Homo sapiens (human) ]
• Official Symbol: NLK
• Synonyms: NLK; nemo-like kinase; nemo like kinase; serine/threonine-protein kinase NLK; FLJ21033; DKFZp761G1211
• Gene ID: 51701
• mRNA Refseq: NM_016231
• Protein Refseq: NP_057315
• MIM: 609476
• UniProt ID: Q9UBE8