Species : |
Human |
Source : |
Insect Cells |
Tag : |
GST&His |
Protein Length : |
121-527 a.a. |
Form : |
Supplied as sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% glyPlease contact us for any concerns or special requirements.Please refer to the specific buffer information in the hard copy of CoA. |
Bio-activity : |
The specific activity was determined to be 3 nmol/min/mg using MBP as substrate. |
Molecular Mass : |
The recombinant human NLK /GST chimera consists of 644 amino acids and has a calculated molecular mass of 74.1 kDa. The recombinant protein migrates as an approximately 73 kDa band in SDS-PAGE under reducing conditions. |
Proteinlength : |
Val121-Glu527 |
Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method |
Purity : |
> 91 % as determined by SDS-PAGE |
Storage : |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |