Recombinant Full Length Human PCNA Protein, C-Flag-tagged

• Cat.No.: PCNA-249HFL
• Product Overview: Recombinant Full Length Human PCNA Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 28.6 kDa
• AA Sequence: MFEARLVQGSILKKVLEALKDLINE ACWDISSSGVNLQSMDSSHVSLVQL TLRSEGFDTYRCDRNLAMGV NLTSMSKILKCAGNEDIITLRAEDN ADTLALVFEAPNQEKVSDYEMKLMD LDVEQLGIPEQEYSCVVKMP SGEFARICRDLSHIGDAVVISCAKD GVKFSASGELGNGNIKLSQTSNVDK EEEAVTIEMNEPVQLTFALR
  YLNFFTKATPLSSTVTLSMSADVPL VVEYKIADMGHLKYYLAPKIEDEEG STRTRPLEQKLISEEDLAANDILDY KDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Base excision repair, Cell cycle, DNA replication, Mismatch repair, Nucleotide excision repair

Gene Information

• Gene Name: PCNA proliferating cell nuclear antigen [ Homo sapiens (human) ]
• Official Symbol: PCNA
• Synonyms: ATLD2
• Gene ID: 5111
• mRNA Refseq: NM_002592.2
• Protein Refseq: NP_002583.1
• MIM: 176740
• UniProt ID: P12004