Human POLG Knockdown Cell Lysate

• Cat.No.: POLG-509HKCL
• Product Overview: WB-validated POLG Knockdown HT-1080 Cell Lysate

Specification

• Species: Human
• Source: HT-1080
• Tag: Non
• Description: Mitochondrial DNA polymerase is heterotrimeric, consisting of a homodimer of accessory subunits plus a catalytic subunit. The protein encoded by this gene is the catalytic subunit of mitochondrial DNA polymerase. The encoded protein contains a polyglutamine tract near its N-terminus that may be polymorphic. Defects in this gene are a cause of progressive external ophthalmoplegia with mitochondrial DNA deletions 1 (PEOA1), sensory ataxic neuropathy dysarthria and ophthalmoparesis (SANDO), Alpers-Huttenlocher syndrome (AHS), and mitochondrial neurogastrointestinal encephalopathy syndrome (MNGIE). Two transcript variants encoding the same protein have been found for this gene.
• Form: Cell-Tissue Lysis buffer
• Molecular Mass: 140 kDa
• Notes: Instruction of use: This knockdown cell lysate should be paired with wild-type HT-1080 cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins.
• Storage: Store at -20 centigrade for two years.
• Concentration: Lot-specific
• Shipping: Blue Ice
• Components: 1 vial of 100 μg WT HT-1080 cell lysate 1 vial of 100 μg POLG KD HT-1080 cell lysate
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways
• Lysate QC: RT-qPCR; Western Blotting (WB)

Gene Information

• Gene Name: POLG polymerase (DNA directed), gamma [ Homo sapiens (human) ]
• Official Symbol: POLG
• Synonyms: POLG; polymerase (DNA directed), gamma; DNA polymerase subunit gamma-1; POLG1; POLGA; PolG-alpha; PolG, catalytic subunit; mitochondrial DNA polymerase catalytic subunit; PEO; MDP1; SCAE; MIRAS; SANDO; MTDPS4A; MTDPS4B; FLJ27114
• Gene ID: 5428
• mRNA Refseq: NM_001126131
• Protein Refseq: NP_001119603
• MIM: 174763
• UniProt ID: P54098