Recombinant Full Length Rat POLG Protein, His-Avi-tagged

• Cat.No.: POLG-4225R
• Product Overview: Recombinant full-length Rat POLG (DNA Polymerase Gamma, Catalytic Subunit) Protein, His-Avi-tagged, expressed in Baculovirus-Insect Cells (1-1216 aa).

Specification

• Species: Rat
• Source: Baculovirus-Insect Cells
• Tag: Avi&His
• Protein Length: 1-1216 aa
• Description: POLG encodes the catalytic subunit of mitochondrial DNA polymerase, responsible for replication and repair of mitochondrial DNA. The enzyme possesses both DNA polymerase and 3'-5' exonuclease proofreading activities. It is essential for mtDNA maintenance. Mutations in POLG are associated with a spectrum of mitochondrial disorders including progressive external ophthalmoplegia (PEO), Alpers syndrome, and mitochondrial neurogastrointestinal encephalopathy (MNGIE).
• Form: Liquid
• Molecular Mass: 138 kDa (calculated)
• AASequence: MGSSHHHHHHSSGLNDIFEAQKIEWHEGGGGSVSSSVLDPVPSDGQPQSQMPSSENGQLRLNPLHIQMLSRGLHEQIFGCGGDVPDEAAVQRSIEHLRKHGLWGQPTTPLPDVQLRLPRLFGGNLDQHFRLLAQKQSLPYLEAAASLSEAQLPPQPRKWVWAEGWTRYGPEGEAEPVAIPEERALVFDVEVCLAEGTCPTLAVAISPSAWYSWCSRRLVEERYSWTSQLSPADLIPLGVSASASSSTQQDWQEQLVVGHNVSFDRAHIREQYLIQGSRMRFLDTMSMHMAISGLSSFQRSLWMGAKQGKHKTQHPTKRGQKSQKNANGPAISSWDWMDISSANNLADVHNLYVGGPRLAKEPRELFVKGSMRDIRENFQDLMEYCARDVWATFEVFQQQLPLFLERCPHPVTLAGMLEMGVSYLPVNQNWERYLTEAQSTYEELQREMKKSLMELANDACQLLSGERYKEDPWLWDLEWDLQEFKQKKAKKVKKTASASKLPIEGAGPFGDPMDQEDPGPPSEEEELQQNIMAHTRLQQLKSTTDLLPKRPQHLPGHPGWYRKLCPRLDDPAWTPGPSLLSLQMRVTPKLMALTWDGFPLHYSDSHGWGYLVPGRRDNLTELPVSPTEESAAVTCPYRAIESLYRRHCLDQGKQQLETQETDLAEEFLLTDSAMWQTVEELGCLDVEAEATVESSGLSQPLVPPTACAPKTSQPTYHHGNGPYNDVDIPGCWFFKLPHKDGNNYNVGSPFAKDFLPKMEDGTLQAGPGGARGPRALEINKMISFWRNAHKRISSQMVVWLPRSALPRAVTRHPSFDEESHYGAILPQVVTAGTITRRAVEPTWLTASNARPDRVGSELKAMVQAPPGYVLVGADVDSQELWIAAVLGDAHFAGMHGCTAFGWMTLQGRKSRGTDLHSKTAATVGISREHAKVFNYGRIYGAGQSFAERLLMQFNHRLSRQEAADKAQQMYAVTKGLRRYRLSDDGEWLVKQLNVPVDRTEDGWVSLQDLRKIRREASRKSRWKKWEVVTERAWTGGTESEMFNKLESIAMSDTPRTPVLGCCISRALEPSVVQGEFMTSRVNWVVQSSAVDYLHLMLVAMKWLFEEFAIDGRFCISIHDEVRYLVREEDRYRAALALQITNLLTRCMFAYKLGLNDLPQSVAFFSAVDIDQCLRKEVTMDCKTPSNPTGMERKYGIPQGEALDIYQIIELTKGSLEKRSQPGP
• Endotoxin: <1 EU/ug by LAL
• Purity: >85% by SDS-PAGE
• Storage: -20 to -80C. Store it under sterile conditions. Aliquot for optimal storage. Avoid repeated freeze-thaw cycles.
• Concentration: 0.3 mg/ml by BCA
• Storage Buffer: Sterile PBS, pH7.4

Gene Information

• Gene Name: POLG
• Official Symbol: Polg
• Synonyms: POLG;POLG1;POLGA;SCAE;MDP1;PEO;MTDPS4A;MTDPS4B
• Gene ID: 85472
• mRNA Refseq: NM_053528.2
• Protein Refseq: NP_445980.2
• MIM: 174763
• UniProt ID: Q9QYV8