Human PPP3CA Knockdown Cell Lysate

• Cat.No.: PPP3CA-275HKCL
• Product Overview: WB-validated PPP3CA Knockdown HeLa Cell Lysate

Specification

• Species: Human
• Source: HeLa
• Tag: Non
• Description: Enables several functions, including ATPase binding activity; calmodulin binding activity; and calmodulin-dependent protein phosphatase activity. Involved in several processes, including calcineurin-NFAT signaling cascade; negative regulation of angiotensin-activated signaling pathway; and peptidyl-serine dephosphorylation. Located in cytoplasm; cytoplasmic side of plasma membrane; and dendritic spine. Part of calcineurin complex. Implicated in developmental and epileptic encephalopathy 91. Biomarker of cholangiocarcinoma; focal segmental glomerulosclerosis; and schizophrenia.
• Form: Cell-Tissue Lysis buffer
• Molecular Mass: 59 kDa
• Notes: Instruction of use: This knockdown cell lysate should be paired with wild-type HeLa cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins.
• Storage: Store at -20 centigrade for two years.
• Concentration: Lot-specific
• Shipping: Blue Ice
• Components: 1 vial of 100 μg WT HeLa cell lysate 1 vial of 100 μg PPP3CA KD HeLa cell lysate
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Axon guidance, B cell receptor signaling pathway, Calcium signaling pathway, Long-term potentiation, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, Oocyte meiosis, T cell receptor signaling pathway, VEGF signaling pathway, Wnt signaling pathway
• Lysate QC: RT-qPCR; Western Blotting (WB)

Gene Information

• Gene Name: PPP3CA protein phosphatase 3, catalytic subunit, alpha isozyme [ Homo sapiens (human) ]
• Official Symbol: PPP3CA
• Synonyms: PPP3CA; protein phosphatase 3, catalytic subunit, alpha isozyme; CALN, CALNA, protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform, protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform (calcineurin A alpha); serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform; calcineurin A alpha; CNA1; PPP2B; protein phosphatase 2B; catalytic subunit; alpha isoform; CAM-PRP catalytic subunit; calmodulin-dependent calcineurin A subunit alpha isoform; protein phosphatase 2B, catalytic subunit, alpha isoform; protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform; CALN; CCN1; CALNA; CALNA1
• Gene ID: 5530
• mRNA Refseq: NM_000944
• Protein Refseq: NP_000935
• MIM: 114105
• UniProt ID: Q08209