| Species : |
Human |
| Source : |
HT-1080 |
| Tag : |
Non |
| Description : |
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene. |
| Form : |
Cell-Tissue Lysis buffer |
| Molecular Mass : |
52 kDa |
| Notes : |
Instruction of use: This knockdown cell lysate should be paired with wild-type HT-1080 cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins. |
| Storage : |
Store at -20 centigrade. |
| Concentration : |
Lot-specific |
| Shipping : |
Blue Ice |
| Components : |
1 vial of 100 μg WT HT-1080 cell lysate
1 vial of 100 μg SMAD2 KD HT-1080 cell lysate |
| Protein Families : |
Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Stem cell relevant signaling - JAK/STAT signaling pathway, Stem cell relevant signaling - TGFb/BMP signaling pathway, Transcription Factors |
| Protein Pathways : |
Adherens junction, Cell cycle, Colorectal cancer, Pancreatic cancer, Pathways in cancer, TGF-beta signaling pathway, Wnt signaling pathway |
| Lysate QC : |
RT-qPCR; Western Blotting (WB) |
