Human SMAD5 Knockdown Cell Lysate

• Cat.No.: SMAD5-296HKCL
• Product Overview: WB-validated SMAD5 Knockdown HeLa Cell Lysate

Specification

• Species: Human
• Source: HeLa
• Tag: Non
• Description: The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants.
• Form: Cell-Tissue Lysis buffer
• Molecular Mass: 52 kDa
• Notes: Instruction of use: This knockdown cell lysate should be paired with wild-type HeLa cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins.
• Storage: Store at -20 centigrade for two years.
• Concentration: Lot-specific
• Shipping: Blue Ice
• Components: 1 vial of 100 μg WT HeLa cell lysate 1 vial of 100 μg SMAD5 KD HeLa cell lysate
• Protein Families: Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Stem cell relevant signaling - JAK/STAT signaling pathway, Stem cell relevant signaling - TGFb/BMP signaling pathway, Transcription Factors
• Protein Pathways: TGF-beta signaling pathway
• Lysate QC: RT-qPCR; Western Blotting (WB)

Gene Information

• Gene Name: SMAD5 SMAD family member 5 [ Homo sapiens (human) ]
• Official Symbol: SMAD5
• Synonyms: SMAD5; SMAD family member 5; MAD, mothers against decapentaplegic homolog 5 (Drosophila), MADH5, SMAD, mothers against DPP homolog 5 (Drosophila); mothers against decapentaplegic homolog 5; Dwfc; JV5 1; SMA- and MAD-related protein 5; SMAD, mothers against DPP homolog 5; MAD, mothers against decapentaplegic homolog 5; mothers against decapentaplegic, drosophila, homolog of, 5; DWFC; JV5-1; MADH5; DKFZp781C1895; DKFZp781O1323
• Gene ID: 4090
• mRNA Refseq: NM_001001419
• Protein Refseq: NP_001001419
• MIM: 603110
• UniProt ID: Q99717