| Species : |
Human |
| Source : |
HeLa |
| Tag : |
Non |
| Description : |
This gene is a tumor suppressor gene that encodes the growth inhibitory protein tuberin. Tuberin interacts with hamartin to form the TSC protein complex which functions in the control of cell growth. This TSC protein complex negatively regulates mammalian target of rapamycin complex 1 (mTORC1) signaling which is a major regulator of anabolic cell growth. Mutations in this gene have been associated with tuberous sclerosis and lymphangioleiomyomatosis. |
| Form : |
Cell-Tissue Lysis buffer |
| Molecular Mass : |
201 kDa |
| Notes : |
Instruction of use: This knockdown cell lysate should be paired with wild-type HeLa cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins. |
| Storage : |
Store at -20 centigrade for two years. |
| Concentration : |
Lot-specific |
| Shipping : |
Blue Ice |
| Components : |
1 vial of 100 μg WT HeLa cell lysate
1 vial of 100 μg TSC2 KD HeLa cell lysate |
| Protein Families : |
Druggable Genome |
| Protein Pathways : |
Insulin signaling pathway, mTOR signaling pathway, p53 signaling pathway |
| Lysate QC : |
RT-qPCR; Western Blotting (WB) |
