Human U2AF2 Knockdown Cell Lysate

• Cat.No.: U2AF2-460HKCL
• Product Overview: WB-validated U2AF2 Knockdown HeLa Cell Lysate

Specification

• Species: Human
• Source: HeLa
• Tag: Non
• Description: U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date.
• Form: Cell-Tissue Lysis buffer
• Molecular Mass: 54 kDa
• Notes: Instruction of use: This knockdown cell lysate should be paired with wild-type HeLa cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins.
• Storage: Store at -20 centigrade for two years.
• Concentration: Lot-specific
• Shipping: Blue Ice
• Components: 1 vial of 100 μg WT HeLa cell lysate 1 vial of 100 μg U2AF2 KD HeLa cell lysate
• Protein Pathways: Spliceosome
• Lysate QC: RT-qPCR; Western Blotting (WB)

Gene Information

• Gene Name: U2AF2 U2 small nuclear RNA auxiliary factor 2 [ Homo sapiens (human) ]
• Official Symbol: U2AF2
• Synonyms: U2AF2; U2 small nuclear RNA auxiliary factor 2; U2 (RNU2) small nuclear RNA auxiliary factor 2; splicing factor U2AF 65 kDa subunit; splicing factor U2AF 65 kD subunit; U2 small nuclear ribonucleoprotein auxiliary factor (65kD); U2 snRNP auxiliary factor large subunit; U2AF65; hU2AF65; hU2AF(65); U2 auxiliary factor 65 kDa subunit
• Gene ID: 11338
• mRNA Refseq: NM_001012478
• Protein Refseq: NP_001012496
• MIM: 191318
• UniProt ID: P26368