Human UBE4B Knockdown Cell Lysate

• Cat.No.: UBE4B-462HKCL
• Product Overview: WB-validated UBE4B Knockdown HeLa Cell Lysate

Specification

• Species: Human
• Source: HeLa
• Tag: Non
• Description: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly. This gene is also the strongest candidate in the neuroblastoma tumor suppressor genes. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
• Form: Cell-Tissue Lysis buffer
• Molecular Mass: 146 kDa
• Notes: Instruction of use: This knockdown cell lysate should be paired with wild-type HeLa cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins.
• Storage: Store at -20 centigrade for two years.
• Concentration: Lot-specific
• Shipping: Blue Ice
• Components: 1 vial of 100 μg WT HeLa cell lysate 1 vial of 100 μg UBE4B KD HeLa cell lysate
• Protein Pathways: Ubiquitin mediated proteolysis
• Lysate QC: RT-qPCR; Western Blotting (WB)

Gene Information

• Gene Name: UBE4B ubiquitination factor E4B [ Homo sapiens (human) ]
• Official Symbol: UBE4B
• Synonyms: UBE4B; ubiquitination factor E4B; ubiquitination factor E4B (homologous to yeast UFD2), ubiquitination factor E4B (UFD2 homolog, yeast); ubiquitin conjugation factor E4 B; E4; KIAA0684; UBOX3; UFD2; homologous to yeast UFD2; UFD2A-III/UBE4B-III splice isoform; ubiquitin fusion degradation protein 2; ubiquitin-fusion degradation protein 2; homozygously deleted in neuroblastoma 1; homozygously deleted in neuroblastoma-1; ubiquitination factor E4B (UFD2 homolog, yeast); ubiquitination factor E4B (homologous to yeast UFD2); HDNB1; UFD2A
• Gene ID: 10277
• mRNA Refseq: NM_001105562
• Protein Refseq: NP_001099032
• MIM: 613565
• UniProt ID: O95155