Immobilized Bovine Mucin protein


Home / Products / Others / Others / Immobilized Bovine Mucin protein

Immobilized Bovine Mucin protein

Mucin Related Products

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

Cat.No. : Mucin-336BI
Product Overview : Submaxillary Mucin was from Bovine. The bead size is of 50-250 micron and the spacer is 3 carbon atom arm. The linkage between spacer and bead is imidoester.
Description : Immobilized carbohydrates and glycoproteins have been used successfully for the selective adsorption of proteins (e.g. lectins) which have an affinity for the carbohydrate or glycoprotein that has been covalently linked to support a matrix. Purification may be carried out by applying a sample to the gel, washing through any unbound material, and eluting with a specific carbohydrate solution. The eluting carbohydrate can be removed either by dialysis or gel filtration. The gel is washed adequately to re-use the column many times. The amount of material that can be isolated in each experimental run will vary greatly and is dependent upon the molecular weight of the protein being isolated, its affinity for the particular carbohydrate linked to the gel, flow rates, and temperature.
The gel matrix is polygalactose which may cause problems with proteins having a specificity for galactose.
Source : Bovine
Species : Bovine
Form : 0.01M Phosphate - 0.15M NaCl, pH 7.2-7.4 Contains 0.05% sodium azide as a preservative.
Usage : The following information is provided only for your convenience. The success of your experiments are not guaranteed.
Procedure for Use:
1. The reaction may be carried out in a test tube (which will require centrifugation for washing steps) or in a small column (either a glass pipette or a plastic mini-column.) Gels may be run at room temperature or in a cold room. Elevated temperatures should be avoided.
2. Wash gel with 10 times the gel volume using the appropriate Buffer. Many proteins will require different buffers, pH, and ionic conditions for binding. Many will also require the addition of specific ions to insure binding. These conditions must be determined, or discovered, experimentally by the researcher.
3. Apply sample and wash unbound material from column with Buffer of your choice. DO NOT OVERWASH! The affinity for the carbohydrate/glycoprotein depends on the binding constant of the protein. Extensive washing may elute the protein to be purified if the binding constant is low.
4. Elute bound material using the appropriate carbohydrate in the Buffer of your choice. Collect small samples. Unless the optimal carbohydrate concentration has been previously determined it is recommended to start with 0.1M - 0.2M.

Procedure for Gel Regeneration:
1. After elution, wash the gel with 10 times the gel volume using 1.0-1.4 M NaCl in distilled water. Re-equilibrate the gel by washing with 50 times the gel volume using the Storage Buffer. Store refrigerated with 0.05% sodium azide as a preservative. DO NOT store the column in the high salt concentration solution. DO NOT FREEZE.
Stability : The material is stable for several years when stored refrigerated with 0.05% sodium azide added as a preservative.
Storage : Store refrigerated at 5-8 °C in Buffer. DO NOT FREEZE.
Official Symbol : Mucin
Synonyms : Mucin
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Online Inquiry

  • Note: There will be extra charge for optional service!
  • Verification Code Please review Creative BioMart's privacy policy for more information

Optional Service: Optional requirements on this protein

Other Requirements:

Apply For A Coupon

$50 OFF Your First Purchase

Apply For a Coupon

Enter your email here to subscribe.

creative biomart inc.

Easy access to products and services you need from our library via powerful searching tools.

Follow Us

Copyright © 2021 Creative BioMart. All Rights Reserved. Terms and Conditions | Privacy Policy